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3qf6

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Revision as of 01:24, 5 August 2016

Neutron structure of type-III Antifreeze Protein allows the reconstruction of AFP-ice interface

Structural highlights

3qf6 is a 1 chain structure with sequence from Blennius americanus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	1hg7
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ANP12_ZOAAM] Contributes to protect fish blood from freezing at subzero sea water temperatures. Lowers the blood freezing point. Binds to nascent ice crystals and prevents further growth.

Publication Abstract from PubMed

Antifreeze proteins (AFPs) inhibit ice growth at sub-zero temperatures. The prototypical type-III AFPs have been extensively studied, notably by X-ray crystallography, solid-state and solution NMR, and mutagenesis, leading to the identification of a compound ice-binding surface (IBS) composed of two adjacent ice-binding sections, each which binds to particular lattice planes of ice crystals, poisoning their growth. This surface, including many hydrophobic and some hydrophilic residues, has been extensively used to model the interaction of AFP with ice. Experimentally observed water molecules facing the IBS have been used in an attempt to validate these models. However, these trials have been hindered by the limited capability of X-ray crystallography to reliably identify all water molecules of the hydration layer. Due to the strong diffraction signal from both the oxygen and deuterium atoms, neutron diffraction provides a more effective way to determine the water molecule positions (as D(2) O). Here we report the successful structure determination at 293 K of fully perdeuterated type-III AFP by joint X-ray and neutron diffraction providing a very detailed description of the protein and its solvent structure. X-ray data were collected to a resolution of 1.05 A, and neutron Laue data to a resolution of 1.85 A with a "radically small" crystal volume of 0.13 mm(3). The identification of a tetrahedral water cluster in nuclear scattering density maps has allowed the reconstruction of the IBS-bound ice crystal primary prismatic face. Analysis of the interactions between the IBS and the bound ice crystal primary prismatic face indicates the role of the hydrophobic residues, which are found to bind inside the holes of the ice surface, thus explaining the specificity of AFPs for ice versus water.

Neutron structure of type-III antifreeze protein allows the reconstruction of AFP-ice interface.,Howard EI, Blakeley MP, Haertlein M, Petit-Haertlein I, Mitschler A, Fisher SJ, Cousido-Siah A, Salvay AG, Popov A, Muller-Dieckmann C, Petrova T, Podjarny A J Mol Recognit. 2011 Jul-Aug;24(4):724-32. doi: 10.1002/jmr.1130. PMID:21472814^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Howard EI, Blakeley MP, Haertlein M, Petit-Haertlein I, Mitschler A, Fisher SJ, Cousido-Siah A, Salvay AG, Popov A, Muller-Dieckmann C, Petrova T, Podjarny A. Neutron structure of type-III antifreeze protein allows the reconstruction of AFP-ice interface. J Mol Recognit. 2011 Jul-Aug;24(4):724-32. doi: 10.1002/jmr.1130. PMID:21472814 doi:10.1002/jmr.1130

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3qf6"

@@ Line 1: / Line 1: @@
 ==Neutron structure of type-III Antifreeze Protein allows the reconstruction of AFP-ice interface==
 <StructureSection load='3qf6' size='340' side='right' caption='[[3qf6]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3qf6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Zoarces_americanus Zoarces americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QF6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QF6 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3qf6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Blennius_americanus Blennius americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QF6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QF6 FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DOD:DEUTERATED+WATER'>DOD</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1hg7|1hg7]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qf6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qf6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qf6 RCSB], [http://www.ebi.ac.uk/pdbsum/3qf6 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qf6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qf6 OCA], [http://pdbe.org/3qf6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3qf6 RCSB], [http://www.ebi.ac.uk/pdbsum/3qf6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3qf6 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/ANP12_MACAM ANP12_MACAM]] Contributes to protect fish blood from freezing at subzero sea water temperatures. Lowers the blood freezing point. Binds to nascent ice crystals and prevents further growth.
+[[http://www.uniprot.org/uniprot/ANP12_ZOAAM ANP12_ZOAAM]] Contributes to protect fish blood from freezing at subzero sea water temperatures. Lowers the blood freezing point. Binds to nascent ice crystals and prevents further growth.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3qf6" style="background-color:#fffaf0;"></div>
 ==See Also==
@@ Line 24: / Line 26: @@
 __TOC__
 </StructureSection>
-[[Category: Zoarces americanus]]
+[[Category: Blennius americanus]]
 [[Category: Blakeley, M P]]
 [[Category: Cousido-Siah, A]]

3qf6

From Proteopedia

Revision as of 01:24, 5 August 2016

Neutron structure of type-III Antifreeze Protein allows the reconstruction of AFP-ice interface

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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