This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

2y3e

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 03:29, 5 August 2016

TRAPTAVIDIN, APO-FORM

Structural highlights

2y3e is a 2 chain structure with sequence from As 4.1583. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	1swh, 2bc3, 1vwq, 1swf, 1sre, 1lcw, 2rtb, 1swp, 1hxz, 1rxj, 1swe, 1mm9, 2rtr, 1swa, 2rto, 2rtd, 1stp, 1n43, 1sri, 2izh, 1slf, 1swc, 1vwr, 1swl, 1ndj, 1str, 1vwm, 1slg, 1swd, 1swq, 1swu, 1i9h, 2rtj, 1swj, 2rtl, 2iza, 1vwk, 1vwn, 1vwg, 1moy, 1sts, 1hqq, 1vwi, 2izg, 2izb, 1swg, 1luq, 1swk, 2izk, 2y3f, 1sws, 1n4j, 2izd, 1swt, 1lcv, 1df8, 1vwl, 2wpu, 1vwb, 1srj, 2rtk, 1kl3, 2rti, 2izf, 1swr, 2rth, 2rtm, 2izi, 1vwf, 1vwh, 1pts, 1mk5, 1hxl, 2f01, 1vwd, 1nc9, 1sle, 1vwj, 2ize, 2rtf, 2rtp, 1n9y, 1n9m, 1lcz, 1nqm, 1hy2, 1vwa, 2izl, 2izc, 1kl5, 1mep, 1sld, 1rxh, 1rsu, 1vwc, 1nbx, 2izj, 1srh, 1rst, 1kff, 1srg, 1vwe, 2rtg, 1srf, 1kl4, 1rxk, 2rtq, 1vwo, 1swb, 2rte, 2rta, 2rtc, 1swo, 2rtn, 1swn, 1n7y, 1vwp
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).

Publication Abstract from PubMed

The interaction between streptavidin and biotin is one of the strongest non-covalent interactions in nature. Streptavidin is a widely used tool and a paradigm for protein-ligand interactions. We recently developed a streptavidin mutant, termed traptavidin, possessing 10-fold lower off-rate for biotin, with increased mechanical and thermal stability. Here, we determined crystal structures of apo-traptavidin and biotin-traptavidin at 1.5 A resolution. In apo-streptavidin the L3/4 loop, near biotin's valeryl tail, is typically disordered and open, but closes upon biotin binding. In contrast, this L3/4 loop was shut in both apo-traptavidin and biotin-traptavidin. The reduced flexibility of L3/4 and decreased conformational change on biotin binding provide an explanation for traptavidin's reduced biotin off-rate and on-rate. The L3/4 loop includes Ser-45, which forms a hydrogen bond to biotin consistently in traptavidin but erratically in streptavidin. Reduced breakage of the biotin:Ser-45 hydrogen bond in traptavidin is likely to inhibit the initiating event in biotin's dissociation pathway. We generated a traptavidin with 1 biotin binding site rather than 4, which showed a similarly slow off-rate, demonstrating that traptavidin's slow off-rate was governed by intra-subunit effects. Understanding the structural features of this tenacious interaction may assist design of even stronger affinity tags and inhibitors.

How the biotin-streptavidin interaction was made even stronger: investigation via crystallography and a chimeric tetramer.,Chivers CE, Koner AL, Lowe ED, Howarth M Biochem J. 2011 Jan 18. PMID:21241253^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chivers CE, Koner AL, Lowe ED, Howarth M. How the biotin-streptavidin interaction was made even stronger: investigation via crystallography and a chimeric tetramer. Biochem J. 2011 Jan 18. PMID:21241253 doi:10.1042/BJ20101593

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2y3e"

@@ Line 1: / Line 1: @@
 ==TRAPTAVIDIN, APO-FORM==
 <StructureSection load='2y3e' size='340' side='right' caption='[[2y3e]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2y3e]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y3E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2Y3E FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2y3e]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y3E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2Y3E FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1swh|1swh]], [[2bc3|2bc3]], [[1vwq|1vwq]], [[1swf|1swf]], [[1sre|1sre]], [[1lcw|1lcw]], [[2rtb|2rtb]], [[1swp|1swp]], [[1hxz|1hxz]], [[1rxj|1rxj]], [[1swe|1swe]], [[1mm9|1mm9]], [[2rtr|2rtr]], [[1swa|1swa]], [[2rto|2rto]], [[2rtd|2rtd]], [[1stp|1stp]], [[1n43|1n43]], [[1sri|1sri]], [[2izh|2izh]], [[1slf|1slf]], [[1swc|1swc]], [[1vwr|1vwr]], [[1swl|1swl]], [[1ndj|1ndj]], [[1str|1str]], [[1vwm|1vwm]], [[1slg|1slg]], [[1swd|1swd]], [[1swq|1swq]], [[1swu|1swu]], [[1i9h|1i9h]], [[2rtj|2rtj]], [[1swj|1swj]], [[2rtl|2rtl]], [[2iza|2iza]], [[1vwk|1vwk]], [[1vwn|1vwn]], [[1vwg|1vwg]], [[1moy|1moy]], [[1sts|1sts]], [[1hqq|1hqq]], [[1vwi|1vwi]], [[2izg|2izg]], [[2izb|2izb]], [[1swg|1swg]], [[1luq|1luq]], [[1swk|1swk]], [[2izk|2izk]], [[2y3f|2y3f]], [[1sws|1sws]], [[1n4j|1n4j]], [[2izd|2izd]], [[1swt|1swt]], [[1lcv|1lcv]], [[1df8|1df8]], [[1vwl|1vwl]], [[2wpu|2wpu]], [[1vwb|1vwb]], [[1srj|1srj]], [[2rtk|2rtk]], [[1kl3|1kl3]], [[2rti|2rti]], [[2izf|2izf]], [[1swr|1swr]], [[2rth|2rth]], [[2rtm|2rtm]], [[2izi|2izi]], [[1vwf|1vwf]], [[1vwh|1vwh]], [[1pts|1pts]], [[1mk5|1mk5]], [[1hxl|1hxl]], [[2f01|2f01]], [[1vwd|1vwd]], [[1nc9|1nc9]], [[1sle|1sle]], [[1vwj|1vwj]], [[2ize|2ize]], [[2rtf|2rtf]], [[2rtp|2rtp]], [[1n9y|1n9y]], [[1n9m|1n9m]], [[1lcz|1lcz]], [[1nqm|1nqm]], [[1hy2|1hy2]], [[1vwa|1vwa]], [[2izl|2izl]], [[2izc|2izc]], [[1kl5|1kl5]], [[1mep|1mep]], [[1sld|1sld]], [[1rxh|1rxh]], [[1rsu|1rsu]], [[1vwc|1vwc]], [[1nbx|1nbx]], [[2izj|2izj]], [[1srh|1srh]], [[1rst|1rst]], [[1kff|1kff]], [[1srg|1srg]], [[1vwe|1vwe]], [[2rtg|2rtg]], [[1srf|1srf]], [[1kl4|1kl4]], [[1rxk|1rxk]], [[2rtq|2rtq]], [[1vwo|1vwo]], [[1swb|1swb]], [[2rte|2rte]], [[2rta|2rta]], [[2rtc|2rtc]], [[1swo|1swo]], [[2rtn|2rtn]], [[1swn|1swn]], [[1n7y|1n7y]], [[1vwp|1vwp]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2y3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y3e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2y3e RCSB], [http://www.ebi.ac.uk/pdbsum/2y3e PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2y3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y3e OCA], [http://pdbe.org/2y3e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2y3e RCSB], [http://www.ebi.ac.uk/pdbsum/2y3e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2y3e ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 2y3e" style="background-color:#fffaf0;"></div>
 ==See Also==
@@ Line 24: / Line 26: @@
 __TOC__
 </StructureSection>
-[[Category: Streptomyces avidinii]]
+[[Category: As 4 1583]]
 [[Category: Chivers, C E]]
 [[Category: Howarth, M]]

2y3e

From Proteopedia

Revision as of 03:29, 5 August 2016

TRAPTAVIDIN, APO-FORM

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox