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3zc7

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Revision as of 07:50, 5 August 2016

VbhT Fic protein from Bartonella schoenbuchensis in complex with VbhA antitoxin and ATP

Structural highlights

3zc7 is a 2 chain structure with sequence from Bartonella schoenbuchensis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[VBHT_BARSR] Toxic component of a toxin-antitoxin (TA) module VbhT-VbhA. Adenylyltransferase involved in virulence by mediating the addition of adenosine 5'-monophosphate (AMP) to specific residue of host GTPases. The resulting AMPylation affects GTPases, impairing actin assembly in infected cells. [VBHA_BARSR] Antitoxin component of a toxin-antitoxin (TA) module VbhT-VbhA. Acts by inhibiting the adenylyltransferase activity of VbhT; competes with ATP-binding and prevents productive ATP-binding to VbhT.

Publication Abstract from PubMed

The ubiquitous FIC domain is evolutionarily conserved from bacteria to human and has been shown to catalyze AMP transfer onto protein side-chain hydroxyl groups. Recently, it was predicted that most catalytically competent Fic proteins are inhibited by the presence of an inhibitory helix alphainh that is provided by a cognate anti-toxin (class I), or is part of the N- or C-terminal part of the Fic protein itself (classes II and III). In vitro, inhibition is relieved by mutation of a conserved glutamate of alphainh to glycine. For the class III bacterial Fic protein NmFic from Neisseria meningitidis, the inhibitory mechanism has been elucidated. Here, we extend above study by including bacterial class I and II Fic proteins VbhT from Bartonella schoenbuchensis and SoFic from Shewanella oneidensis, respectively, and the respective E->G mutants. Comparative enzymatic and crystallographic analyses show that, in all three classes, the ATP substrate binds to the wild-type FIC domains, but with the alpha-phosphate in disparate and non-competent orientations. In the E->G mutants, however, the tri-phosphate moiety is found reorganized to the same tightly bound structure through a unique set of hydrogen bonds with Fic signature motif residues. The gamma-phosphate adopts the location that is taken by the inhibitory glutamate in wild-type resulting in an alpha-phosphate orientation that can be attacked in-line by a target side-chain hydroxyl group. The latter is properly registered to the Fic active center by main-chain beta-interactions with the beta-hairpin flap. These data indicate that the active site motif and the exposed edge of the flap are both required to form an adenylylation-competent Fic protein.

Conserved inhibitory mechanism and competent ATP binding mode for adenylyltransferases with fic fold.,Goepfert A, Stanger FV, Dehio C, Schirmer T PLoS One. 2013 May 30;8(5):e64901. doi: 10.1371/journal.pone.0064901. Print 2013. PMID:23738009^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Goepfert A, Stanger FV, Dehio C, Schirmer T. Conserved inhibitory mechanism and competent ATP binding mode for adenylyltransferases with fic fold. PLoS One. 2013 May 30;8(5):e64901. doi: 10.1371/journal.pone.0064901. Print 2013. PMID:23738009 doi:10.1371/journal.pone.0064901

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3zc7"

@@ Line 1: / Line 1: @@
 ==VbhT Fic protein from Bartonella schoenbuchensis in complex with VbhA antitoxin and ATP==
 <StructureSection load='3zc7' size='340' side='right' caption='[[3zc7]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
@@ Line 4: / Line 5: @@
 <table><tr><td colspan='2'>[[3zc7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bartonella_schoenbuchensis Bartonella schoenbuchensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZC7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZC7 FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zc7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zc7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zc7 RCSB], [http://www.ebi.ac.uk/pdbsum/3zc7 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zc7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zc7 OCA], [http://pdbe.org/3zc7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zc7 RCSB], [http://www.ebi.ac.uk/pdbsum/3zc7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zc7 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 16: / Line 17: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3zc7" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

3zc7

From Proteopedia

Revision as of 07:50, 5 August 2016

VbhT Fic protein from Bartonella schoenbuchensis in complex with VbhA antitoxin and ATP

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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