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4c4w

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Revision as of 09:15, 5 August 2016

Structure of a rare, non-standard sequence k-turn bound by L7Ae protein

Structural highlights

4c4w is a 8 chain structure with sequence from Arcfu and Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	4c40
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SNRPA_HUMAN] Binds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process. Binds preferentially to the 5'-UGCAC-3' motif in vitro.^[1] [RL7A_ARCFU] Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, box H/ACA and box C/D sRNAs.^[2]

Publication Abstract from PubMed

Kt-23 from Thelohania solenopsae is a rare RNA kink turn (k-turn) where an adenine replaces the normal guanine at the 2n position. L7Ae is a member of a strongly conserved family of proteins that bind a range of k-turn structures in the ribosome, box C/D and H/ACA small nucleolar RNAs and U4 small nuclear RNA. We have solved the crystal structure of T. solenopsae Kt-23 RNA bound to Archeoglobus fulgidus L7Ae protein at a resolution of 2.95 A. The protein binds in the major groove displayed on the outer face of the k-turn, in a manner similar to complexes with standard k-turn structures. The k-turn adopts a standard N3 class conformation, with a single hydrogen bond from A2b N6 to A2n N3. This contrasts with the structure of the same sequence located in the SAM-I riboswitch, where it adopts an N1 structure, showing the inherent plasticity of k-turn structure. This potentially can affect any tertiary interactions in which the RNA participates.

Structure of a rare non-standard sequence k-turn bound by L7Ae protein.,Huang L, Lilley DM Nucleic Acids Res. 2014 Jan 29. PMID:24482444^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lutz CS, Cooke C, O'Connor JP, Kobayashi R, Alwine JC. The snRNP-free U1A (SF-A) complex(es): identification of the largest subunit as PSF, the polypyrimidine-tract binding protein-associated splicing factor. RNA. 1998 Dec;4(12):1493-9. PMID:9848648
↑ Rozhdestvensky TS, Tang TH, Tchirkova IV, Brosius J, Bachellerie JP, Huttenhofer A. Binding of L7Ae protein to the K-turn of archaeal snoRNAs: a shared RNA binding motif for C/D and H/ACA box snoRNAs in Archaea. Nucleic Acids Res. 2003 Feb 1;31(3):869-77. PMID:12560482
↑ Huang L, Lilley DM. Structure of a rare non-standard sequence k-turn bound by L7Ae protein. Nucleic Acids Res. 2014 Jan 29. PMID:24482444 doi:http://dx.doi.org/10.1093/nar/gku087

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4c4w"

@@ Line 1: / Line 1: @@
 ==Structure of a rare, non-standard sequence k-turn bound by L7Ae protein==
 <StructureSection load='4c4w' size='340' side='right' caption='[[4c4w]], [[Resolution|resolution]] 2.95&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2HP:DIHYDROGENPHOSPHATE+ION'>2HP</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4c40|4c40]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c4w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c4w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4c4w RCSB], [http://www.ebi.ac.uk/pdbsum/4c4w PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c4w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c4w OCA], [http://pdbe.org/4c4w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4c4w RCSB], [http://www.ebi.ac.uk/pdbsum/4c4w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4c4w ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4c4w" style="background-color:#fffaf0;"></div>
 ==See Also==

4c4w

From Proteopedia

Revision as of 09:15, 5 August 2016

Structure of a rare, non-standard sequence k-turn bound by L7Ae protein

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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