1mhp
From Proteopedia
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|PDB= 1mhp |SIZE=350|CAPTION= <scene name='initialview01'>1mhp</scene>, resolution 2.8Å | |PDB= 1mhp |SIZE=350|CAPTION= <scene name='initialview01'>1mhp</scene>, resolution 2.8Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=MN:MANGANESE (II) ION'>MN</scene> | + | |LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mhp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mhp OCA], [http://www.ebi.ac.uk/pdbsum/1mhp PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1mhp RCSB]</span> | ||
}} | }} | ||
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[[Category: Taylor, F.]] | [[Category: Taylor, F.]] | ||
[[Category: Weinreb, P.]] | [[Category: Weinreb, P.]] | ||
| - | [[Category: MN]] | ||
[[Category: antibody]] | [[Category: antibody]] | ||
[[Category: cell adhesion]] | [[Category: cell adhesion]] | ||
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[[Category: receptor]] | [[Category: receptor]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:16:14 2008'' |
Revision as of 19:16, 30 March 2008
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| , resolution 2.8Å | |||||||
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| Ligands: | |||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of a chimeric alpha1 integrin I-domain in complex with the Fab fragment of a humanized neutralizing antibody
Overview
The alpha1beta1 (VLA-1) integrin is a cell-surface receptor for collagen and laminin and has been implicated in biological pathways involved in several pathological processes. These processes may be inhibited by the monoclonal antibody AQC2, which binds with high affinity to human alpha1beta1 integrin. To understand the structural basis of the inhibition we determined the crystal structure of the complex of a chimeric rat/human I domain of the alpha1beta1 integrin and the Fab fragment of humanized AQC2 antibody. The structure of the complex shows that the antibody blocks the collagen binding site of the I domain. An aspartate residue, from the CDR3 loop of the antibody heavy chain, coordinates the MIDAS metal ion in a manner similar to that of a glutamate residue from collagen. Substitution of the aspartate residue by alanine or arginine results in significant reduction of antibody binding affinity. Interestingly, although the mode of metal ion coordination resembles that of the open conformation, the I domain maintains an overall closed conformation previously observed only for unliganded I domains.
About this Structure
1MHP is a Single protein structure of sequence from Mus musculus and Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Crystal structure of the alpha1beta1 integrin I domain in complex with an antibody Fab fragment., Karpusas M, Ferrant J, Weinreb PH, Carmillo A, Taylor FR, Garber EA, J Mol Biol. 2003 Apr 11;327(5):1031-41. PMID:12662928
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