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3j6g

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Revision as of 15:54, 5 August 2016

Minimized average structure of microtubules stabilized by taxol

Structural highlights

3j6g is a 18 chain structure with sequence from Sus scrofa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , ,
Related:	3j6e, 3j6f
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[TBA1A_PIG] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [TBB_PIG] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Publication Abstract from PubMed

Dynamic instability, the stochastic switching between growth and shrinkage, is essential for microtubule function. This behavior is driven by GTP hydrolysis in the microtubule lattice and is inhibited by anticancer agents like Taxol. We provide insight into the mechanism of dynamic instability, based on high-resolution cryo-EM structures (4.7-5.6 A) of dynamic microtubules and microtubules stabilized by GMPCPP or Taxol. We infer that hydrolysis leads to a compaction around the E-site nucleotide at longitudinal interfaces, as well as movement of the alpha-tubulin intermediate domain and H7 helix. Displacement of the C-terminal helices in both alpha- and beta-tubulin subunits suggests an effect on interactions with binding partners that contact this region. Taxol inhibits most of these conformational changes, allosterically inducing a GMPCPP-like state. Lateral interactions are similar in all conditions we examined, suggesting that microtubule lattice stability is primarily modulated at longitudinal interfaces.

High-Resolution Microtubule Structures Reveal the Structural Transitions in alphabeta-Tubulin upon GTP Hydrolysis.,Alushin GM, Lander GC, Kellogg EH, Zhang R, Baker D, Nogales E Cell. 2014 May 22;157(5):1117-29. doi: 10.1016/j.cell.2014.03.053. PMID:24855948^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Alushin GM, Lander GC, Kellogg EH, Zhang R, Baker D, Nogales E. High-Resolution Microtubule Structures Reveal the Structural Transitions in alphabeta-Tubulin upon GTP Hydrolysis. Cell. 2014 May 22;157(5):1117-29. doi: 10.1016/j.cell.2014.03.053. PMID:24855948 doi:http://dx.doi.org/10.1016/j.cell.2014.03.053

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3j6g"

@@ Line 1: / Line 1: @@
 ==Minimized average structure of microtubules stabilized by taxol==
 <StructureSection load='3j6g' size='340' side='right' caption='[[3j6g]], [[Resolution|resolution]] 5.50&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TA1:TAXOL'>TA1</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3j6e|3j6e]], [[3j6f|3j6f]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j6g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j6g RCSB], [http://www.ebi.ac.uk/pdbsum/3j6g PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j6g OCA], [http://pdbe.org/3j6g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j6g RCSB], [http://www.ebi.ac.uk/pdbsum/3j6g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j6g ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3j6g" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Tubulin|Tubulin]]
 == References ==
 <references/>

3j6g

From Proteopedia

Revision as of 15:54, 5 August 2016

Minimized average structure of microtubules stabilized by taxol

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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