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3c2g

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Revision as of 18:01, 5 August 2016

Crystal complex of SYS-1/POP-1 at 2.5A resolution

Structural highlights

3c2g is a 4 chain structure with sequence from Caenorhabditis elegans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:
Related:	3c2h
Gene:	SYS-1 (Caenorhabditis elegans), POP-1 (Caenorhabditis elegans)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[POP1_CAEEL] Part of the Wnt signaling pathway essential for the specification of the mesodermal cell fate in early embryos. Required for asymmetrical division of somatic gonadal precursor descendants which initiate axis formation required to control organ shape. Represses expression of target genes via its interaction with hda-1 histone deacetylase.^[1] ^[2]

Publication Abstract from PubMed

C. elegans SYS-1 has key functional characteristics of a canonical beta-catenin, but no significant sequence similarity. Here, we report the SYS-1 crystal structure, both on its own and in a complex with POP-1, the C. elegans TCF homolog. The two structures possess signature features of canonical beta-catenin and the beta-catenin/TCF complex that could not be predicted by sequence. Most importantly, SYS-1 bears 12 armadillo repeats and the SYS-1/POP-1 interface is anchored by a conserved salt-bridge, the "charged button." We also modeled structures for three other C. elegans beta-catenins to predict the molecular basis of their distinct binding properties. Finally, we generated a phylogenetic tree, using the region of highest structural similarity between SYS-1 and beta-catenin, and found that SYS-1 clusters robustly within the beta-catenin clade. We conclude that the SYS-1 protein belongs to the beta-catenin family and suggest that additional divergent beta-catenins await discovery.

The C. elegans SYS-1 protein is a bona fide beta-catenin.,Liu J, Phillips BT, Amaya MF, Kimble J, Xu W Dev Cell. 2008 May;14(5):751-61. PMID:18477457^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Calvo D, Victor M, Gay F, Sui G, Luke MP, Dufourcq P, Wen G, Maduro M, Rothman J, Shi Y. A POP-1 repressor complex restricts inappropriate cell type-specific gene transcription during Caenorhabditis elegans embryogenesis. EMBO J. 2001 Dec 17;20(24):7197-208. PMID:11742996 doi:http://dx.doi.org/10.1093/emboj/20.24.7197
↑ Siegfried KR, Kimble J. POP-1 controls axis formation during early gonadogenesis in C. elegans. Development. 2002 Jan;129(2):443-53. PMID:11807036
↑ Liu J, Phillips BT, Amaya MF, Kimble J, Xu W. The C. elegans SYS-1 protein is a bona fide beta-catenin. Dev Cell. 2008 May;14(5):751-61. PMID:18477457 doi:http://dx.doi.org/S1534-5807(08)00109-3

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3c2g"

@@ Line 1: / Line 1: @@
 ==Crystal complex of SYS-1/POP-1 at 2.5A resolution==
 <StructureSection load='3c2g' size='340' side='right' caption='[[3c2g]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3c2h|3c2h]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SYS-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 Caenorhabditis elegans]), POP-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 Caenorhabditis elegans])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c2g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3c2g RCSB], [http://www.ebi.ac.uk/pdbsum/3c2g PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c2g OCA], [http://pdbe.org/3c2g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3c2g RCSB], [http://www.ebi.ac.uk/pdbsum/3c2g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3c2g ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3c2g" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

3c2g

From Proteopedia

Revision as of 18:01, 5 August 2016

Crystal complex of SYS-1/POP-1 at 2.5A resolution

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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