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3cw0

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Revision as of 19:44, 5 August 2016

E.coli DmsD

Structural highlights

3cw0 is a 4 chain structure with sequence from Ecoli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	dmsD, ynfI (ECOLI)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[DMSD_ECOLI] Required for biogenesis/assembly of DMSO reductase, but not for the interaction of the DmsA signal peptide with the Tat system. May be part of a chaperone cascade complex that facilitates a folding-maturation pathway for the substrate protein.^[1] ^[2] ^[3] ^[4]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The translocation of folded proteins via the twin-arginine translocation (Tat) pathway is regulated to prevent the futile export of inactive substrate. DmsD is part of a class of cytoplasmic chaperones that play a role in preventing certain redox proteins from premature transport. DmsD from Escherichia coli has been crystallized in space group P4(1)2(1)2, with unit-cell parameters a = b = 97.45, c = 210.04 A, in the presence of a small peptide. The structure has been solved by molecular replacement to a resolution of 2.4 A and refined to an R factor of 19.4%. There are four molecules in the asymmetric unit that may mimic a higher order structure in vivo. There appears to be density for the peptide in a predicted binding pocket, which lends support to its role as the signal-recognition surface for this class of proteins.

Structure of the twin-arginine signal-binding protein DmsD from Escherichia coli.,Ramasamy SK, Clemons WM Jr Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Aug 1;65(Pt, 8):746-50. Epub 2009 Jul 21. PMID:19652330^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Oresnik IJ, Ladner CL, Turner RJ. Identification of a twin-arginine leader-binding protein. Mol Microbiol. 2001 Apr;40(2):323-31. PMID:11309116
↑ Ray N, Oates J, Turner RJ, Robinson C. DmsD is required for the biogenesis of DMSO reductase in Escherichia coli but not for the interaction of the DmsA signal peptide with the Tat apparatus. FEBS Lett. 2003 Jan 16;534(1-3):156-60. PMID:12527378
↑ Papish AL, Ladner CL, Turner RJ. The twin-arginine leader-binding protein, DmsD, interacts with the TatB and TatC subunits of the Escherichia coli twin-arginine translocase. J Biol Chem. 2003 Aug 29;278(35):32501-6. Epub 2003 Jun 17. PMID:12813051 doi:http://dx.doi.org/10.1074/jbc.M301076200
↑ Li H, Chang L, Howell JM, Turner RJ. DmsD, a Tat system specific chaperone, interacts with other general chaperones and proteins involved in the molybdenum cofactor biosynthesis. Biochim Biophys Acta. 2010 Jun;1804(6):1301-9. doi: 10.1016/j.bbapap.2010.01.022., Epub 2010 Feb 11. PMID:20153451 doi:http://dx.doi.org/10.1016/j.bbapap.2010.01.022
↑ Ramasamy SK, Clemons WM Jr. Structure of the twin-arginine signal-binding protein DmsD from Escherichia coli. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Aug 1;65(Pt, 8):746-50. Epub 2009 Jul 21. PMID:19652330 doi:10.1107/S1744309109023811

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3cw0"

@@ Line 1: / Line 1: @@
 ==E.coli DmsD==
 <StructureSection load='3cw0' size='340' side='right' caption='[[3cw0]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
@@ Line 4: / Line 5: @@
 <table><tr><td colspan='2'>[[3cw0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CW0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CW0 FirstGlance]. <br>
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">dmsD, ynfI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cw0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3cw0 RCSB], [http://www.ebi.ac.uk/pdbsum/3cw0 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cw0 OCA], [http://pdbe.org/3cw0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3cw0 RCSB], [http://www.ebi.ac.uk/pdbsum/3cw0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3cw0 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 16: / Line 17: @@
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
-</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cw0 ConSurf].
 <div style="clear:both"></div>
 <div style="background-color:#fffaf0;">
@@ Line 26: / Line 27: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3cw0" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

3cw0

From Proteopedia

Revision as of 19:44, 5 August 2016

E.coli DmsD

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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