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4m8o

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Revision as of 22:12, 5 August 2016

TERNARY COMPLEX OF DNA POLYMERASE EPSILON WITH AN INCOMING dATP

Structural highlights

4m8o is a 3 chain structure with sequence from Baker's yeast. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , , ,
NonStd Res:	, ,
Gene:	POL2, DUN2, YNL262W, N0825 (Baker's yeast)
Activity:	DNA-directed DNA polymerase, with EC number 2.7.7.7
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[DPOE_YEAST] DNA polymerase epsilon (DNA polymerase II) participates in chromosomal DNA replication. It is required during synthesis of the leading and lagging DNA strands at the replication fork and binds at/or near replication origins and moves along DNA with the replication fork. It has 3'-5' proofreading exonuclease activity that correct errors arising during DNA replication. It is also involved in DNA synthesis during DNA repair.^[1]

Publication Abstract from PubMed

DNA polymerase varepsilon (Pol varepsilon) is a high-fidelity polymerase that has been shown to participate in leading-strand synthesis during DNA replication in eukaryotic cells. We present here a ternary structure of the catalytic core of Pol varepsilon (142 kDa) from Saccharomyces cerevisiae in complex with DNA and an incoming nucleotide. This structure provides information about the selection of the correct nucleotide and the positions of amino acids that might be critical for proofreading activity. Pol varepsilon has the highest fidelity among B-family polymerases despite the absence of an extended beta-hairpin loop that is required for high-fidelity replication by other B-family polymerases. Moreover, the catalytic core has a new domain that allows Pol varepsilon to encircle the nascent double-stranded DNA. Altogether, the structure provides an explanation for the high processivity and high fidelity of leading-strand DNA synthesis in eukaryotes.

Structural basis for processive DNA synthesis by yeast DNA polymerase varepsilon.,Hogg M, Osterman P, Bylund GO, Ganai RA, Lundstrom EB, Sauer-Eriksson AE, Johansson E Nat Struct Mol Biol. 2014 Jan;21(1):49-55. doi: 10.1038/nsmb.2712. Epub 2013 Dec , 1. PMID:24292646^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Shimizu K, Hashimoto K, Kirchner JM, Nakai W, Nishikawa H, Resnick MA, Sugino A. Fidelity of DNA polymerase epsilon holoenzyme from budding yeast Saccharomyces cerevisiae. J Biol Chem. 2002 Oct 4;277(40):37422-9. Epub 2002 Jul 17. PMID:12124389 doi:http://dx.doi.org/10.1074/jbc.M204476200
↑ Hogg M, Osterman P, Bylund GO, Ganai RA, Lundstrom EB, Sauer-Eriksson AE, Johansson E. Structural basis for processive DNA synthesis by yeast DNA polymerase varepsilon. Nat Struct Mol Biol. 2014 Jan;21(1):49-55. doi: 10.1038/nsmb.2712. Epub 2013 Dec , 1. PMID:24292646 doi:http://dx.doi.org/10.1038/nsmb.2712

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4m8o"

@@ Line 1: / Line 1: @@
 ==TERNARY COMPLEX OF DNA POLYMERASE EPSILON WITH AN INCOMING dATP==
 <StructureSection load='4m8o' size='340' side='right' caption='[[4m8o]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
@@ Line 7: / Line 8: @@
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">POL2, DUN2, YNL262W, N0825 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m8o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4m8o RCSB], [http://www.ebi.ac.uk/pdbsum/4m8o PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m8o OCA], [http://pdbe.org/4m8o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4m8o RCSB], [http://www.ebi.ac.uk/pdbsum/4m8o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4m8o ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 19: / Line 20: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4m8o" style="background-color:#fffaf0;"></div>
 ==See Also==

4m8o

From Proteopedia

Revision as of 22:12, 5 August 2016

TERNARY COMPLEX OF DNA POLYMERASE EPSILON WITH AN INCOMING dATP

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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