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1n4l
From Proteopedia
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|PDB= 1n4l |SIZE=350|CAPTION= <scene name='initialview01'>1n4l</scene>, resolution 2.00Å | |PDB= 1n4l |SIZE=350|CAPTION= <scene name='initialview01'>1n4l</scene>, resolution 2.00Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/RNA-directed_DNA_polymerase RNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.49 2.7.7.49] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_DNA_polymerase RNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.49 2.7.7.49] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1doe|1DOE]], [[1d1u|1D1U]], [[1i6j|1I6J]], [[1qai|1QAI]], [[1qaj|1QAJ]], [[1n85|1N85]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n4l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n4l OCA], [http://www.ebi.ac.uk/pdbsum/1n4l PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1n4l RCSB]</span> | ||
}} | }} | ||
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[[Category: Georgiadis, M M.]] | [[Category: Georgiadis, M M.]] | ||
[[Category: Pflomm, M.]] | [[Category: Pflomm, M.]] | ||
| - | [[Category: mmlv rt | + | [[Category: asymmetric dna]] |
| + | [[Category: hiv-1]] | ||
| + | [[Category: mmlv rt]] | ||
| + | [[Category: polypurine tract]] | ||
| + | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:25:17 2008'' |
Revision as of 19:25, 30 March 2008
| |||||||
| , resolution 2.00Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Activity: | RNA-directed DNA polymerase, with EC number 2.7.7.49 | ||||||
| Related: | 1DOE, 1D1U, 1I6J, 1QAI, 1QAJ, 1N85
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
A DNA analogue of the polypurine tract of HIV-1
Overview
The polypurine tract (PPT) from the HIV-1 genome is resistant to digestion by reverse transcriptase following (-)-strand synthesis and is used to prime (+)-strand synthesis during retroviral replication. We have determined the crystal structure of the asymmetric DNA/DNA analog16-mer duplex (CTTTTTAAAAGAAAAG/CTTTTCTTTTAAAAAG) comprising most of the "visible" portion of the RNA:DNA hybrid from the polypurine tract of HIV-1, which was previously reported in a complex with HIV-1 reverse transcriptase. Our 16-mer completely encompasses a 10-mer DNA duplex analog of the HIV-1 PPT. We report here a detailed analysis of our B' form 16-mer DNA structure, including three full pure A-tracts, as well as a comparative structural analysis with polypurine tract and other A-tract-containing nucleic acid structures. Our analysis reveals that the polypurine tract structures share structural features despite being different nucleic acid forms (i.e. DNA:DNA versus RNA:DNA). In addition, the previously reported A-tract-containing DNA molecules bound to topoisomerase I are remarkably similar to our polypurine tract 16-mer structure. On the basis of our analysis, we suggest that the specific topology of long pure A-tracts is remarkably comparable across a wide array of biological environments.
About this Structure
1N4L is a Single protein structure of sequence from Moloney murine leukemia virus. Full crystallographic information is available from OCA.
Reference
Staying straight with A-tracts: a DNA analog of the HIV-1 polypurine tract., Cote ML, Pflomm M, Georgiadis MM, J Mol Biol. 2003 Jun 27;330(1):57-74. PMID:12818202
Page seeded by OCA on Sun Mar 30 22:25:17 2008
