This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

3j8c

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 11:58, 11 August 2016

Model of the human eIF3 PCI-MPN octamer docked into the 43S EM map

Structural highlights

3j8c is a 8 chain structure with sequence from Homo sapiens. This structure supersedes the now removed PDB entry 3j7k. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:
Related:	3j8b, 4u1d, 4u1c, 4lct, 4b4t, 4o8x, 1rz4, 3chm, 3j47
Activity:	Ubiquitinyl hydrolase 1, with EC number 3.4.19.12
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Eukaryotic translation initiation begins with assembly of a 43S preinitiation complex. First, methionylated initiator methionine transfer RNA (Met-tRNAi(Met)), eukaryotic initiation factor (eIF) 2, and guanosine triphosphate form a ternary complex (TC). The TC, eIF3, eIF1, and eIF1A cooperatively bind to the 40S subunit, yielding the 43S preinitiation complex, which is ready to attach to messenger RNA (mRNA) and start scanning to the initiation codon. Scanning on structured mRNAs additionally requires DHX29, a DExH-box protein that also binds directly to the 40S subunit. Here, we present a cryo-electron microscopy structure of the mammalian DHX29-bound 43S complex at 11.6 A resolution. It reveals that eIF2 interacts with the 40S subunit via its alpha subunit and supports Met-tRNAi(Met) in an unexpected P/I orientation (eP/I). The structural core of eIF3 resides on the back of the 40S subunit, establishing two principal points of contact, whereas DHX29 binds around helix 16. The structure provides insights into eukaryote-specific aspects of translation, including the mechanism of action of DHX29.

Structure of the mammalian ribosomal 43S preinitiation complex bound to the scanning factor DHX29.,Hashem Y, des Georges A, Dhote V, Langlois R, Liao HY, Grassucci RA, Hellen CU, Pestova TV, Frank J Cell. 2013 May 23;153(5):1108-19. doi: 10.1016/j.cell.2013.04.036. PMID:23706745^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hashem Y, des Georges A, Dhote V, Langlois R, Liao HY, Grassucci RA, Hellen CU, Pestova TV, Frank J. Structure of the mammalian ribosomal 43S preinitiation complex bound to the scanning factor DHX29. Cell. 2013 May 23;153(5):1108-19. doi: 10.1016/j.cell.2013.04.036. PMID:23706745 doi:http://dx.doi.org/10.1016/j.cell.2013.04.036

1 Structural highlights
2 Publication Abstract from PubMed
3 See Also
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3j8c"

Categories: Homo sapiens | Ubiquitinyl hydrolase 1 | Ban, N | Erzberger, J P | Translation

@@ Line 1: / Line 1: @@
 ==Model of the human eIF3 PCI-MPN octamer docked into the 43S EM map==
 <StructureSection load='3j8c' size='340' side='right' caption='[[3j8c]]' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3j8b|3j8b]], [[4u1d|4u1d]], [[4u1c|4u1c]], [[4lct|4lct]], [[4b4t|4b4t]], [[4o8x|4o8x]], [[1rz4|1rz4]], [[3chm|3chm]], [[3j47|3j47]]</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitinyl_hydrolase_1 Ubiquitinyl hydrolase 1], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.19.12 3.4.19.12] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j8c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j8c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j8c RCSB], [http://www.ebi.ac.uk/pdbsum/3j8c PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j8c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j8c OCA], [http://pdbe.org/3j8c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j8c RCSB], [http://www.ebi.ac.uk/pdbsum/3j8c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j8c ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 16: / Line 17: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3j8c" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Eukaryotic initiation factor|Eukaryotic initiation factor]]
 == References ==
 <references/>

3j8c

From Proteopedia

Revision as of 11:58, 11 August 2016

Model of the human eIF3 PCI-MPN octamer docked into the 43S EM map

Structural highlights

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox