1nyb
From Proteopedia
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|PDB= 1nyb |SIZE=350|CAPTION= <scene name='initialview01'>1nyb</scene> | |PDB= 1nyb |SIZE=350|CAPTION= <scene name='initialview01'>1nyb</scene> | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene> |
|ACTIVITY= | |ACTIVITY= | ||
| - | |GENE= N ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= | + | |GENE= N ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10737 Enterobacteria phage phi21]) |
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1a4t|1A4T]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nyb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nyb OCA], [http://www.ebi.ac.uk/pdbsum/1nyb PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1nyb RCSB]</span> | ||
}} | }} | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1NYB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | + | 1NYB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_phi21 Enterobacteria phage phi21]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NYB OCA]. |
==Reference== | ==Reference== | ||
Structural mimicry in the phage phi21 N peptide-boxB RNA complex., Cilley CD, Williamson JR, RNA. 2003 Jun;9(6):663-76. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12756325 12756325] | Structural mimicry in the phage phi21 N peptide-boxB RNA complex., Cilley CD, Williamson JR, RNA. 2003 Jun;9(6):663-76. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12756325 12756325] | ||
| - | [[Category: | + | [[Category: Enterobacteria phage phi21]] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Cilley, C D.]] | [[Category: Cilley, C D.]] | ||
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[[Category: transcription antitermination]] | [[Category: transcription antitermination]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:37:16 2008'' |
Revision as of 19:37, 30 March 2008
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| Ligands: | , , , | ||||||
| Gene: | N (Enterobacteria phage phi21) | ||||||
| Related: | 1A4T
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
SOLUTION STRUCTURE OF THE BACTERIOPHAGE PHI21 N PEPTIDE-BOXB RNA COMPLEX
Overview
We determined the solution structure of a 22-amino-acid peptide from the amino-terminal domain of the bacteriophage phi21 N protein in complex with its cognate 24-mer boxB RNA hairpin using heteronuclear magnetic resonance spectroscopy. The N peptide binds as an alpha-helix and interacts predominately with the major groove side of the 5' half of the boxB RNA stem-loop. This binding interface is defined by surface complementarity of polar and nonpolar interactions, and little sequence-specific recognition. The phi21 boxB loop (CUAACC) has hydrogen bond and backbone torsions typical of the "U-turn" motif, as well as base stacking of the last 4 nt, and a hydrogen bonded C:C pair closing the loop. The exposed face of the phi21 boxB loop, in complex with the N peptide, is strikingly similar to the GNRA tetraloop-like folds of the related lambda and P22 bacteriophage N peptide-boxB RNA complexes. The N peptide-boxB complexes of the various phage, while individually distinct, provide similar structural features for interactions with the Escherichia coli host factors to enable antitermination.
About this Structure
1NYB is a Single protein structure of sequence from Enterobacteria phage phi21. Full crystallographic information is available from OCA.
Reference
Structural mimicry in the phage phi21 N peptide-boxB RNA complex., Cilley CD, Williamson JR, RNA. 2003 Jun;9(6):663-76. PMID:12756325
Page seeded by OCA on Sun Mar 30 22:37:16 2008
