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3wx5

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Revision as of 16:27, 11 August 2016

Crystal structure of metagenome-derived glycoside hydrolase family 12 endoglucanase

Structural highlights

3wx5 is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Activity:	Cellulase, with EC number 3.2.1.4
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Ten genes encoding novel cellulases with putative signal peptides at the N-terminus, termed pre-LC-CelA-J, were isolated from a fosmid library of a leaf-branch compost metagenome by functional screening using agar plates containing carboxymethyl cellulose and trypan blue. All the cellulases except pre-LC-CelG have a 14-29 residue long flexible linker (FL) between the signal peptide and the catalytic domain. LC-CelA without a signal peptide (residues 20-261), which shows 76% amino acid sequence identity to Cel12A from Rhodothermus marinus (RmCel12A), was overproduced in Escherichia coli, purified and characterized. LC-CelA exhibited its highest activity across a broad pH range (pH 5-9) and at 90 degrees C, indicating that LC-CelA is a highly thermostable cellulase, like RmCel12A. The crystal structure of LC-CelA was determined at 1.85 A resolution and is nearly identical to that of RmCel12A determined in a form without the FL. Both proteins contain two disulfide bonds. LC-CelA has a 16-residue FL (residues 20-35), most of which is not visible in the electron density map, probably due to structural disorder. However, Glu34 and Pro35 form hydrogen bonds with the central region of the protein. DeltaFL-LC-CelA (residues 36-261) and E34A-LC-CelA with a single Glu34 --> Ala mutation were therefore constructed and characterized. DeltaFL-LC-CelA and E34A-LC-CelA had lower melting temperatures (T m) than LC-CelA by 14.7 and 12.0 degrees C respectively. The T m of LC-CelA was also decreased by 28.0 degrees C in the presence of dithiothreitol. These results suggest that Glu34-mediated hydrogen bonds and the two disulfide bonds contribute to the stabilization of LC-CelA.

Structure and stability of metagenome-derived glycoside hydrolase family 12 cellulase (LC-CelA) a homolog of Cel12A from Rhodothermus marinus.,Okano H, Ozaki M, Kanaya E, Kim JJ, Angkawidjaja C, Koga Y, Kanaya S FEBS Open Bio. 2014 Oct 31;4:936-46. doi: 10.1016/j.fob.2014.10.013. eCollection , 2014. PMID:25426413^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Okano H, Ozaki M, Kanaya E, Kim JJ, Angkawidjaja C, Koga Y, Kanaya S. Structure and stability of metagenome-derived glycoside hydrolase family 12 cellulase (LC-CelA) a homolog of Cel12A from Rhodothermus marinus. FEBS Open Bio. 2014 Oct 31;4:936-46. doi: 10.1016/j.fob.2014.10.013. eCollection , 2014. PMID:25426413 doi:http://dx.doi.org/10.1016/j.fob.2014.10.013

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3wx5"

@@ Line 1: / Line 1: @@
 ==Crystal structure of metagenome-derived glycoside hydrolase family 12 endoglucanase==
 <StructureSection load='3wx5' size='340' side='right' caption='[[3wx5]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
@@ Line 4: / Line 5: @@
 <table><tr><td colspan='2'>[[3wx5]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WX5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WX5 FirstGlance]. <br>
 </td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wx5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wx5 RCSB], [http://www.ebi.ac.uk/pdbsum/3wx5 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wx5 OCA], [http://pdbe.org/3wx5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wx5 RCSB], [http://www.ebi.ac.uk/pdbsum/3wx5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wx5 ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 14: / Line 15: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3wx5" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

3wx5

From Proteopedia

Revision as of 16:27, 11 August 2016

Crystal structure of metagenome-derived glycoside hydrolase family 12 endoglucanase

Structural highlights

Publication Abstract from PubMed

References

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