1obc
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1obc |SIZE=350|CAPTION= <scene name='initialview01'>1obc</scene>, resolution 2.10Å | |PDB= 1obc |SIZE=350|CAPTION= <scene name='initialview01'>1obc</scene>, resolution 2.10Å | ||
|SITE= <scene name='pdbsite=AC1:Lms+Binding+Site+For+Chain+A'>AC1</scene> | |SITE= <scene name='pdbsite=AC1:Lms+Binding+Site+For+Chain+A'>AC1</scene> | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=2AD:2'-AMINO-2'-DEOXYADENOSINE'>2AD</scene>, <scene name='pdbligand=LEU:LEUCINE'>LEU</scene>, <scene name='pdbligand=LMS:[(2R,3S,4R,5R)-5-(6-AMINO-9H-PURIN-9-YL)-3,4-DIHYDROXYTETRAHYDRO-2-FURANYL]METHYL+SULFAMATE'>LMS</scene>, <scene name='pdbligand=NVA:NORVALINE'>NVA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1obc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1obc OCA], [http://www.ebi.ac.uk/pdbsum/1obc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1obc RCSB]</span> | ||
}} | }} | ||
| Line 25: | Line 28: | ||
[[Category: Tukalo, M.]] | [[Category: Tukalo, M.]] | ||
[[Category: Yaremchuk, A.]] | [[Category: Yaremchuk, A.]] | ||
| - | [[Category: LEU]] | ||
| - | [[Category: LMS]] | ||
| - | [[Category: ZN]] | ||
[[Category: aminoacyl-trna synthetase]] | [[Category: aminoacyl-trna synthetase]] | ||
[[Category: atp + l-leucine + trna (leu) -> amp + ppi l-leucyl-trna(leu)]] | [[Category: atp + l-leucine + trna (leu) -> amp + ppi l-leucyl-trna(leu)]] | ||
| Line 33: | Line 33: | ||
[[Category: synthetase]] | [[Category: synthetase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:42:43 2008'' |
Revision as of 19:42, 30 March 2008
| |||||||
| , resolution 2.10Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | |||||||
| Ligands: | , , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
LEUCYL-TRNA SYNTHETASE FROM THERMUS THERMOPHILUS COMPLEXED WITH A POST-TRANSFER EDITING SUBSTRATE ANALOGUE
Overview
The aminoacyl-tRNA synthetases link tRNAs with their cognate amino acid. In some cases, their fidelity relies on hydrolytic editing that destroys incorrectly activated amino acids or mischarged tRNAs. We present structures of leucyl-tRNA synthetase complexed with analogs of the distinct pre- and posttransfer editing substrates. The editing active site binds the two different substrates using a single amino acid discriminatory pocket while preserving the same mode of adenine recognition. This suggests a similar mechanism of hydrolysis for both editing substrates that depends on a key, completely conserved aspartic acid, which interacts with the alpha-amino group of the noncognate amino acid and positions both substrates for hydrolysis. Our results demonstrate the economy by which a single active site accommodates two distinct substrates in a proofreading process critical to the fidelity of protein synthesis.
About this Structure
1OBC is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.
Reference
Structural and mechanistic basis of pre- and posttransfer editing by leucyl-tRNA synthetase., Lincecum TL Jr, Tukalo M, Yaremchuk A, Mursinna RS, Williams AM, Sproat BS, Van Den Eynde W, Link A, Van Calenbergh S, Grotli M, Martinis SA, Cusack S, Mol Cell. 2003 Apr;11(4):951-63. PMID:12718881[[Category: atp + l-leucine + trna (leu) -> amp + ppi l-leucyl-trna(leu)]]
Page seeded by OCA on Sun Mar 30 22:42:43 2008
