Riboswitch
From Proteopedia
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| - | + | <StructureSection load='1y26' size='350' side='right' scene='' caption='Adenine riboswitch complex with adenine and Mg+2 ions, [[1y26]]'> | |
== Function == | == Function == | ||
Normally, a variety of proteins and protein cofactors control gene expression in an organism by binding to different sites on messenger RNA (mRNA). '''Riboswitches''' are genetic regulatory elements that are built directly into the RNA. They are a type of noncoding RNA that regulate gene expression in the absence of proteins by switching from one structural conformation (shape) to another in response to ligand binding. Most contain a single binding site that recognizes a specific ligand. The ability of a riboswitch to discriminate against molecules that are similar or closely related to its ligand is essential to prevent metabolic misregulation<ref name=scimag>Breaker, Ronald R. (28 March, 2008). Complex Riboswitches. ''Science'', 319(5871), 1795-1797. doi:[http://dx.doi.org/10.1126/science.1152621 10.1126/science.1152621]</ref>. | Normally, a variety of proteins and protein cofactors control gene expression in an organism by binding to different sites on messenger RNA (mRNA). '''Riboswitches''' are genetic regulatory elements that are built directly into the RNA. They are a type of noncoding RNA that regulate gene expression in the absence of proteins by switching from one structural conformation (shape) to another in response to ligand binding. Most contain a single binding site that recognizes a specific ligand. The ability of a riboswitch to discriminate against molecules that are similar or closely related to its ligand is essential to prevent metabolic misregulation<ref name=scimag>Breaker, Ronald R. (28 March, 2008). Complex Riboswitches. ''Science'', 319(5871), 1795-1797. doi:[http://dx.doi.org/10.1126/science.1152621 10.1126/science.1152621]</ref>. | ||
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== Structural highlights == | == Structural highlights == | ||
Atomic-resolution structures of riboswitch binding sites show that they make numerous hydrogen bonds with their ligands, forming contacts that stabilize RNA interactions to further increase affinity. Some binding sites form pockets that entirely engulf the ligand, and in these instances an induced-fit mechanism of binding must occur. The riboswitch-adenine complex shows the stacking interactions of the zippered-up junctional bubble which is formed by the adenine<ref>PMID:15610857</ref>. | Atomic-resolution structures of riboswitch binding sites show that they make numerous hydrogen bonds with their ligands, forming contacts that stabilize RNA interactions to further increase affinity. Some binding sites form pockets that entirely engulf the ligand, and in these instances an induced-fit mechanism of binding must occur. The riboswitch-adenine complex shows the stacking interactions of the zippered-up junctional bubble which is formed by the adenine<ref>PMID:15610857</ref>. | ||
| - | + | </StructureSection> | |
==3D structures of riboswitch== | ==3D structures of riboswitch== | ||
Revision as of 09:49, 17 August 2016
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3D structures of riboswitch
Updated on 17-August-2016
References
- ↑ Breaker, Ronald R. (28 March, 2008). Complex Riboswitches. Science, 319(5871), 1795-1797. doi:10.1126/science.1152621
- ↑ Serganov A, Yuan YR, Pikovskaya O, Polonskaia A, Malinina L, Phan AT, Hobartner C, Micura R, Breaker RR, Patel DJ. Structural basis for discriminative regulation of gene expression by adenine- and guanine-sensing mRNAs. Chem Biol. 2004 Dec;11(12):1729-41. PMID:15610857 doi:S1074-5521(04)00343-6
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