This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
Single-stranded DNA-binding protein
From Proteopedia
| Line 2: | Line 2: | ||
{{STRUCTURE_1eqq| PDB=1eqq | SIZE=350| SCENE= |right|CAPTION=E. coli SSB complex with ssDNA and UMP (stick model) [[1eqq]] }} | {{STRUCTURE_1eqq| PDB=1eqq | SIZE=350| SCENE= |right|CAPTION=E. coli SSB complex with ssDNA and UMP (stick model) [[1eqq]] }} | ||
| - | '''Single-stranded DNA-binding protein''' (SSB) binds to single-stranded regions of DNA (ssDNA) to protect it from annealing, prevention of secondary structure formation and digestion. SSB binds to DNA in a sequence-independent manner. The binding of SSB enables processes like replication, DNA repair and recombination to occur. SSB can form complexes with specific genome maintenance proteins helping their activities.<br /> | + | '''Single-stranded DNA-binding protein''' (SSB) binds to single-stranded regions of DNA (ssDNA) to protect it from annealing, prevention of secondary structure formation and digestion. SSB binds to DNA in a sequence-independent manner. The binding of SSB enables processes like replication, DNA repair and recombination to occur. SSB can form complexes with specific genome maintenance proteins helping their activities<ref>PMID:22976174</ref>.<br /> |
* '''Viral SSB proteins''' are monomeric.<br /> | * '''Viral SSB proteins''' are monomeric.<br /> | ||
* '''Eukaryotic SSB''' ('''Replication protein A''' – RPA) is a heterotrimer composed of RPA70, RPA32 and RPA14. RPA has 6 DNA-binding domains (DBD).<br /> | * '''Eukaryotic SSB''' ('''Replication protein A''' – RPA) is a heterotrimer composed of RPA70, RPA32 and RPA14. RPA has 6 DNA-binding domains (DBD).<br /> | ||
| Line 77: | Line 77: | ||
**[[1l1o]] – hRPA14 + hRPA32 central domain + hRPA70 C terminal <br /> | **[[1l1o]] – hRPA14 + hRPA32 central domain + hRPA70 C terminal <br /> | ||
}} | }} | ||
| + | == References == | ||
| + | <references/> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Revision as of 09:54, 30 August 2016
Single-stranded DNA-binding protein (SSB) binds to single-stranded regions of DNA (ssDNA) to protect it from annealing, prevention of secondary structure formation and digestion. SSB binds to DNA in a sequence-independent manner. The binding of SSB enables processes like replication, DNA repair and recombination to occur. SSB can form complexes with specific genome maintenance proteins helping their activities[1].
- Viral SSB proteins are monomeric.
- Eukaryotic SSB (Replication protein A – RPA) is a heterotrimer composed of RPA70, RPA32 and RPA14. RPA has 6 DNA-binding domains (DBD).
- Mitochondrial human SSB (HsmtSSB) is a homotetramer.
See also Single stranded binding protein.
3D Structures of single-stranded DNA-binding protein
Updated on 30-August-2016
References
- ↑ Marceau AH. Functions of single-strand DNA-binding proteins in DNA replication, recombination, and repair. Methods Mol Biol. 2012;922:1-21. doi: 10.1007/978-1-62703-032-8_1. PMID:22976174 doi:http://dx.doi.org/10.1007/978-1-62703-032-8_1
