Single-stranded DNA-binding protein
From Proteopedia
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{{STRUCTURE_1eqq| PDB=1eqq | SIZE=350| SCENE= |right|CAPTION=E. coli SSB complex with ssDNA and UMP (stick model) [[1eqq]] }} | {{STRUCTURE_1eqq| PDB=1eqq | SIZE=350| SCENE= |right|CAPTION=E. coli SSB complex with ssDNA and UMP (stick model) [[1eqq]] }} | ||
| - | '''Single-stranded DNA-binding protein''' (SSB) binds to single-stranded regions of DNA (ssDNA) to protect it from annealing, prevention of secondary structure formation and digestion. SSB binds to DNA in a sequence-independent manner. The binding of SSB enables processes like replication, DNA repair and recombination to occur. SSB can form complexes with specific genome maintenance proteins helping their activities.<br /> | + | '''Single-stranded DNA-binding protein''' (SSB) binds to single-stranded regions of DNA (ssDNA) to protect it from annealing, prevention of secondary structure formation and digestion. SSB binds to DNA in a sequence-independent manner. The binding of SSB enables processes like replication, DNA repair and recombination to occur. SSB can form complexes with specific genome maintenance proteins helping their activities<ref>PMID:22976174</ref>.<br /> |
* '''Viral SSB proteins''' are monomeric.<br /> | * '''Viral SSB proteins''' are monomeric.<br /> | ||
* '''Eukaryotic SSB''' ('''Replication protein A''' – RPA) is a heterotrimer composed of RPA70, RPA32 and RPA14. RPA has 6 DNA-binding domains (DBD).<br /> | * '''Eukaryotic SSB''' ('''Replication protein A''' – RPA) is a heterotrimer composed of RPA70, RPA32 and RPA14. RPA has 6 DNA-binding domains (DBD).<br /> | ||
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**[[1l1o]] – hRPA14 + hRPA32 central domain + hRPA70 C terminal <br /> | **[[1l1o]] – hRPA14 + hRPA32 central domain + hRPA70 C terminal <br /> | ||
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| + | == References == | ||
| + | <references/> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Revision as of 09:54, 30 August 2016
Single-stranded DNA-binding protein (SSB) binds to single-stranded regions of DNA (ssDNA) to protect it from annealing, prevention of secondary structure formation and digestion. SSB binds to DNA in a sequence-independent manner. The binding of SSB enables processes like replication, DNA repair and recombination to occur. SSB can form complexes with specific genome maintenance proteins helping their activities[1].
- Viral SSB proteins are monomeric.
- Eukaryotic SSB (Replication protein A – RPA) is a heterotrimer composed of RPA70, RPA32 and RPA14. RPA has 6 DNA-binding domains (DBD).
- Mitochondrial human SSB (HsmtSSB) is a homotetramer.
See also Single stranded binding protein.
3D Structures of single-stranded DNA-binding protein
Updated on 30-August-2016
References
- ↑ Marceau AH. Functions of single-strand DNA-binding proteins in DNA replication, recombination, and repair. Methods Mol Biol. 2012;922:1-21. doi: 10.1007/978-1-62703-032-8_1. PMID:22976174 doi:http://dx.doi.org/10.1007/978-1-62703-032-8_1
