Thiolase
From Proteopedia
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{{STRUCTURE_2iik| PDB=2iik | SIZE=400| SCENE=Thiolase/Thiolase/2 |right|CAPTION=Human 3-ketoacyl-CoA thiolase dimer, [[2iik]] }} | {{STRUCTURE_2iik| PDB=2iik | SIZE=400| SCENE=Thiolase/Thiolase/2 |right|CAPTION=Human 3-ketoacyl-CoA thiolase dimer, [[2iik]] }} | ||
| - | + | == Function == | |
Two different classes of '''thiolases''' are found.<br /> | Two different classes of '''thiolases''' are found.<br /> | ||
| - | *'''Degrative thiolase''' like '''3-ketoacyl-CoA thiolase''' (KCT) is involved in fatty acid β oxidation<ref>PMID:16630629</ref> | + | *'''Degrative thiolase''' like '''3-ketoacyl-CoA thiolase''' (KCT) is involved in fatty acid β oxidation<ref>PMID:16630629</ref>.<br /> |
| - | *'''Biosynthetic thiolases''' like '''acetoacetyl-CoA thiolase''' (ACT) are involved in the thiolysis of acetoacetyl-CoA<ref>PMID:21908473</ref> | + | *'''Biosynthetic thiolases''' like '''acetoacetyl-CoA thiolase''' (ACT) are involved in the thiolysis of acetoacetyl-CoA<ref>PMID:21908473</ref>. |
For Bacterial thiolase see [[Bacterial thiolase]] | For Bacterial thiolase see [[Bacterial thiolase]] | ||
| + | == Structural highlights == | ||
| + | The active site of 3-ketoacyl-CoA thiolase contains CoA<ref>PMID:25478839</ref>. | ||
== 3D Structures of Thiolase == | == 3D Structures of Thiolase == | ||
Revision as of 09:23, 12 September 2016
Contents |
Function
Two different classes of thiolases are found.
- Degrative thiolase like 3-ketoacyl-CoA thiolase (KCT) is involved in fatty acid β oxidation[1].
- Biosynthetic thiolases like acetoacetyl-CoA thiolase (ACT) are involved in the thiolysis of acetoacetyl-CoA[2].
For Bacterial thiolase see Bacterial thiolase
Structural highlights
The active site of 3-ketoacyl-CoA thiolase contains CoA[3].
3D Structures of Thiolase
Updated on 12-September-2016
References
- ↑ Sundaramoorthy R, Micossi E, Alphey MS, Germain V, Bryce JH, Smith SM, Leonard GA, Hunter WN. The crystal structure of a plant 3-ketoacyl-CoA thiolase reveals the potential for redox control of peroxisomal fatty acid beta-oxidation. J Mol Biol. 2006 Jun 2;359(2):347-57. Epub 2006 Mar 29. PMID:16630629 doi:http://dx.doi.org/10.1016/j.jmb.2006.03.032
- ↑ Soto G, Stritzler M, Lisi C, Alleva K, Pagano ME, Ardila F, Mozzicafreddo M, Cuccioloni M, Angeletti M, Ayub ND. Acetoacetyl-CoA thiolase regulates the mevalonate pathway during abiotic stress adaptation. J Exp Bot. 2011 Nov;62(15):5699-711. doi: 10.1093/jxb/err287. Epub 2011 Sep 9. PMID:21908473 doi:http://dx.doi.org/10.1093/jxb/err287
- ↑ Kiema TR, Harijan RK, Strozyk M, Fukao T, Alexson SE, Wierenga RK. The crystal structure of human mitochondrial 3-ketoacyl-CoA thiolase (T1): insight into the reaction mechanism of its thiolase and thioesterase activities. Acta Crystallogr D Biol Crystallogr. 2014 Dec 1;70(Pt 12):3212-25. doi:, 10.1107/S1399004714023827. Epub 2014 Nov 22. PMID:25478839 doi:http://dx.doi.org/10.1107/S1399004714023827
