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5dgk
From Proteopedia
(Difference between revisions)
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dgk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dgk OCA], [http://pdbe.org/5dgk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dgk RCSB], [http://www.ebi.ac.uk/pdbsum/5dgk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5dgk ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dgk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dgk OCA], [http://pdbe.org/5dgk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dgk RCSB], [http://www.ebi.ac.uk/pdbsum/5dgk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5dgk ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Methicillin-resistant Staphylococcus aureus (MRSA) is a public-health threat worldwide. Although the mobile genomic island responsible for this phenotype, staphylococcal cassette chromosome (SCC), has been thought to be nonreplicative, we predicted DNA-replication-related functions for some of the conserved proteins encoded by SCC. We show that one of these, Cch, is homologous to the self-loading initiator helicases of an unrelated family of genomic islands, that it is an active 3'-to-5' helicase and that the adjacent ORF encodes a single-stranded DNA-binding protein. Our 2.9-A crystal structure of intact Cch shows that it forms a hexameric ring. Cch, like the archaeal and eukaryotic MCM-family replicative helicases, belongs to the pre-sensor II insert clade of AAA+ ATPases. Additionally, we found that SCC elements are part of a broader family of mobile elements, all of which encode a replication initiator upstream of their recombinases. Replication after excision would enhance the efficiency of horizontal gene transfer. | ||
| + | |||
| + | Staphylococcal SCCmec elements encode an active MCM-like helicase and thus may be replicative.,Mir-Sanchis I, Roman CA, Misiura A, Pigli YZ, Boyle-Vavra S, Rice PA Nat Struct Mol Biol. 2016 Aug 29. doi: 10.1038/nsmb.3286. PMID:27571176<ref>PMID:27571176</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5dgk" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 08:18, 14 September 2016
SCCmec type IV Cch - active helicase
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