1ou9
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1ou9 |SIZE=350|CAPTION= <scene name='initialview01'>1ou9</scene>, resolution 1.80Å | |PDB= 1ou9 |SIZE=350|CAPTION= <scene name='initialview01'>1ou9</scene>, resolution 1.80Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= SSPB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 Haemophilus influenzae]) | |GENE= SSPB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 Haemophilus influenzae]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1ou8|1OU8]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ou9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ou9 OCA], [http://www.ebi.ac.uk/pdbsum/1ou9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ou9 RCSB]</span> | ||
}} | }} | ||
| Line 27: | Line 30: | ||
[[Category: Sauer, R T.]] | [[Category: Sauer, R T.]] | ||
[[Category: Wah, D A.]] | [[Category: Wah, D A.]] | ||
| - | [[Category: CA]] | ||
[[Category: homodimer]] | [[Category: homodimer]] | ||
[[Category: ssra peptide-binding protein]] | [[Category: ssra peptide-binding protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:50:25 2008'' |
Revision as of 19:50, 30 March 2008
| |||||||
| , resolution 1.80Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | SSPB (Haemophilus influenzae) | ||||||
| Related: | 1OU8
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of SspB, a AAA+ protease delivery protein
Overview
Substrate selection by AAA+ ATPases that function to unfold proteins or alter protein conformation is often regulated by delivery or adaptor proteins. SspB is a protein dimer that binds to the ssrA degradation tag and delivers proteins bearing this tag to ClpXP, an AAA+ protease, for degradation. Here, we describe the structure of the peptide binding domain of H. influenzae SspB in complex with an ssrA peptide at 1.6 A resolution. The ssrA peptides are bound in well-defined clefts located at the extreme ends of the SspB homodimer. SspB contacts residues within the N-terminal and central regions of the 11 residue ssrA tag but leaves the C-terminal residues exposed and positioned to dock with ClpX. This structure, taken together with biochemical analysis of SspB, suggests mechanisms by which proteins like SspB escort substrates to AAA+ ATPases and enhance the specificity and affinity of target recognition.
About this Structure
1OU9 is a Single protein structure of sequence from Haemophilus influenzae. Full crystallographic information is available from OCA.
Reference
Structure of a delivery protein for an AAA+ protease in complex with a peptide degradation tag., Levchenko I, Grant RA, Wah DA, Sauer RT, Baker TA, Mol Cell. 2003 Aug;12(2):365-72. PMID:14536076
Page seeded by OCA on Sun Mar 30 22:50:25 2008
