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Cas9
From Proteopedia
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Revision as of 06:19, 17 September 2016
Cas9 is the RNA-guided DNA endonuclease used by the CRISPR (clustered regularly interspaced short palindromic repeats)-associated systems to generate double-strand DNA breaks in the invading DNA during an adaptive bacterial immune response.
The CRISPR-associated endonuclease Cas9 has been exploited for use in genome editing systems. In such systems, an engineered single-guide RNA (sgRNA) is used to target double-stranded breaks in genomic DNA. Depending on what repair pathway is triggered, often dictated by the inclusion of additional engineered components, the targeted site either is disrupted or incorporates additional genetic sequences.
Move from the McGovern Institute for Brain Research at MIT
Articles in Proteopedia concerning Cas9 include:
Contents |
3D structures of Cas9
Streptococcus pyogenes Cas9
Actinomyces naeslundii Cas9
See Also
References
Proteopedia Page Contributors and Editors (what is this?)
Wayne Decatur, Michal Harel, Karsten Theis, Joel L. Sussman, Ann Taylor, Thomas Gastineau
