1oxf
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1oxf |SIZE=350|CAPTION= <scene name='initialview01'>1oxf</scene>, resolution 1.69Å | |PDB= 1oxf |SIZE=350|CAPTION= <scene name='initialview01'>1oxf</scene>, resolution 1.69Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=4IN:4-AMINO-L-TRYPTOPHAN'>4IN</scene>, <scene name='pdbligand=5ZA:(5Z)-2-[(1S,2R)-1-AMINO-2-HYDROXYPROPYL]-5-[(4-AMINO-1H-INDOL-3-YL)METHYLENE]-3-(2-HYDROXYETHYL)-3,5-DIHYDRO-4H-IMIDAZOL-4-ONE'>5ZA</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1emb|1EMB]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oxf OCA], [http://www.ebi.ac.uk/pdbsum/1oxf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1oxf RCSB]</span> | ||
}} | }} | ||
| Line 16: | Line 19: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1OXF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | + | 1OXF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Cfp_marker_plasmid_pwm1009 Cfp marker plasmid pwm1009]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OXF OCA]. |
==Reference== | ==Reference== | ||
Expansion of the genetic code enables design of a novel "gold" class of green fluorescent proteins., Bae JH, Rubini M, Jung G, Wiegand G, Seifert MH, Azim MK, Kim JS, Zumbusch A, Holak TA, Moroder L, Huber R, Budisa N, J Mol Biol. 2003 May 16;328(5):1071-81. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12729742 12729742] | Expansion of the genetic code enables design of a novel "gold" class of green fluorescent proteins., Bae JH, Rubini M, Jung G, Wiegand G, Seifert MH, Azim MK, Kim JS, Zumbusch A, Holak TA, Moroder L, Huber R, Budisa N, J Mol Biol. 2003 May 16;328(5):1071-81. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12729742 12729742] | ||
| - | [[Category: Cfp marker plasmid | + | [[Category: Cfp marker plasmid pwm1009]] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Azim, M K.]] | [[Category: Azim, M K.]] | ||
| Line 40: | Line 43: | ||
[[Category: tryptophan]] | [[Category: tryptophan]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:51:49 2008'' |
Revision as of 19:51, 30 March 2008
| |||||||
| , resolution 1.69Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Related: | 1EMB
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Expansion of the Genetic Code Enables Design of a Novel "Gold" Class of Green Fluorescent Proteins
Overview
Much effort has been dedicated to the design of significantly red shifted variants of the green fluorescent protein (GFP) from Aequoria victora (av). These approaches have been based on classical engineering with the 20 canonical amino acids. We report here an expansion of these efforts by incorporation of an amino substituted variant of tryptophan into the "cyan" GFP mutant, which turned it into a "gold" variant. This variant possesses a red shift in emission unprecedented for any avFP, similar to "red" FPs, but with enhanced stability and a very low aggregation tendency. An increasing number of non-natural amino acids are available for chromophore redesign (by engineering of the genetic code) and enable new general strategies to generate novel classes of tailor-made GFP proteins.
About this Structure
1OXF is a Single protein structure of sequence from Cfp marker plasmid pwm1009. Full crystallographic information is available from OCA.
Reference
Expansion of the genetic code enables design of a novel "gold" class of green fluorescent proteins., Bae JH, Rubini M, Jung G, Wiegand G, Seifert MH, Azim MK, Kim JS, Zumbusch A, Holak TA, Moroder L, Huber R, Budisa N, J Mol Biol. 2003 May 16;328(5):1071-81. PMID:12729742
Page seeded by OCA on Sun Mar 30 22:51:49 2008
Categories: Cfp marker plasmid pwm1009 | Single protein | Azim, M K. | Bae, J Hyun. | Budisa, N. | Holak, T A. | Huber, R. | Jung, G. | Kim, J S. | Moroder, L. | Rubini, M. | Seifert, M H. | Wiegand, G. | Zumbusch, A. | Amino acid incorporation | Chromophore | Genetic code | Green fluorescent protein | Tryptophan
