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Transposase

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Line 43: Line 43:
**[[1muh]], [[1mus]], [[1mm8]], [[3ecp]], [[4dm0]] – EcTn5 (mutant) + DNA – ''Escherichia coli''<br />
**[[1muh]], [[1mus]], [[1mm8]], [[3ecp]], [[4dm0]] – EcTn5 (mutant) + DNA – ''Escherichia coli''<br />
 +
 +
*Tn7 transposase
 +
 +
**[[5d17]] – EcTn7 C terminal <br />
 +
**[[5d16]] – EcTn7 C terminal (mutant) <br />
*Mos1 transposase
*Mos1 transposase
**[[2f7t]] – DmMos1 catalytic domain (mutant) – ''Drosophila mauritiana''<br />
**[[2f7t]] – DmMos1 catalytic domain (mutant) – ''Drosophila mauritiana''<br />
-
**[[4u7b]] – DmMos1 + DNA <br />
+
**[[4u7b]], [[5hoo]] – DmMos1 + DNA <br />
**[[3hos]], [[3hot]], [[4r79]] – DmMos1 (mutant) + DNA <br />
**[[3hos]], [[3hot]], [[4r79]] – DmMos1 (mutant) + DNA <br />
**[[4mda]], [[4mdb]] – DmMos1 catalytic domain (mutant) + drug<br />
**[[4mda]], [[4mdb]] – DmMos1 catalytic domain (mutant) + drug<br />
Line 59: Line 64:
**[[2a6m]] – HpOrfa – ''Helicobacter pylori''<br />
**[[2a6m]] – HpOrfa – ''Helicobacter pylori''<br />
**[[2vhg]], [[2a6o]], [[2vic]], [[2vih]], [[2vju]], [[2vjv]] – HpOrfa + DNA <br />
**[[2vhg]], [[2a6o]], [[2vic]], [[2vih]], [[2vju]], [[2vjv]] – HpOrfa + DNA <br />
 +
 +
*Sleeping beauty transposase
 +
 +
**[[5cr4]] – SB catalytic domain – synthetic<br />
 +
}}
}}

Revision as of 10:25, 21 September 2016

Structure of Tn5 transposase complex with DNA and glycerol (PDB code 3ecp).

Drag the structure with the mouse to rotate

3D structures of transposase

Updated on 21-September-2016

References

  1. ↑ Ivics Z, Li MA, Mates L, Boeke JD, Nagy A, Bradley A, Izsvak Z. Transposon-mediated genome manipulation in vertebrates. Nat Methods. 2009 Jun;6(6):415-22. doi: 10.1038/nmeth.1332. PMID:19478801 doi:http://dx.doi.org/10.1038/nmeth.1332
  2. ↑ Rice P, Mizuuchi K. Structure of the bacteriophage Mu transposase core: a common structural motif for DNA transposition and retroviral integration. Cell. 1995 Jul 28;82(2):209-20. PMID:7628012
  3. ↑ Park JM, Evertts AG, Levin HL. The Hermes transposon of Musca domestica and its use as a mutagen of Schizosaccharomyces pombe. Methods. 2009 Nov;49(3):243-7. doi: 10.1016/j.ymeth.2009.05.004. Epub 2009 May, 18. PMID:19450689 doi:http://dx.doi.org/10.1016/j.ymeth.2009.05.004
  4. ↑ Reznikoff WS. Transposon Tn5. Annu Rev Genet. 2008;42:269-86. PMID:18680433 doi:http://dx.doi.org/10.1146/annurev.genet.42.110807.091656
  5. ↑ Wang W, Swevers L, Iatrou K. Mariner (Mos1) transposase and genomic integration of foreign gene sequences in Bombyx mori cells. Insect Mol Biol. 2000 Apr;9(2):145-55. PMID:10762422
  6. ↑ Colloms SD, van Luenen HG, Plasterk RH. DNA binding activities of the Caenorhabditis elegans Tc3 transposase. Nucleic Acids Res. 1994 Dec 25;22(25):5548-54. PMID:7838706
  7. ↑ Ivics Z, Kaufman CD, Zayed H, Miskey C, Walisko O, Izsvak Z. The Sleeping Beauty transposable element: evolution, regulation and genetic applications. Curr Issues Mol Biol. 2004 Jan;6(1):43-55. PMID:14632258
  8. ↑ Klenchin VA, Czyz A, Goryshin IY, Gradman R, Lovell S, Rayment I, Reznikoff WS. Phosphate coordination and movement of DNA in the Tn5 synaptic complex: role of the (R)YREK motif. Nucleic Acids Res. 2008 Oct;36(18):5855-62. Epub 2008 Sep 12. PMID:18790806 doi:10.1093/nar/gkn577

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