This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
5leg
From Proteopedia
(Difference between revisions)
m (Protected "5leg" [edit=sysop:move=sysop]) |
|||
| Line 1: | Line 1: | ||
| - | ''' | + | {{Large structure}} |
| + | ==Structure of the bacterial sex F pilus (pED208)== | ||
| + | <StructureSection load='5leg' size='340' side='right' caption='[[5leg]], [[Resolution|resolution]] 3.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5leg]] is a 80 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhi Salmonella enterica subsp. enterica serovar typhi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LEG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LEG FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LHG:1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE'>LHG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5leg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5leg OCA], [http://pdbe.org/5leg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5leg RCSB], [http://www.ebi.ac.uk/pdbsum/5leg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5leg ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | {{Large structure}} | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/PIL1_SALTI PIL1_SALTI]] Propilin is the precursor of the pilus subunit, pilin, that forms conjugative pili, the filamentous surface appendages required for cell-to-cell contact during the earlier stages of bacterial conjugation, and that retract after contact is established. Mature pilin is assembled with the help of TraQ and TraX (By similarity). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Conjugative pili are widespread bacterial appendages that play important roles in horizontal gene transfer, in spread of antibiotic resistance genes, and as sites of phage attachment. Among conjugative pili, the F "sex" pilus encoded by the F plasmid is the best functionally characterized, and it is also historically the most important, as the discovery of F-plasmid-mediated conjugation ushered in the era of molecular biology and genetics. Yet, its structure is unknown. Here, we present atomic models of two F family pili, the F and pED208 pili, generated from cryoelectron microscopy reconstructions at 5.0 and 3.6 A resolution, respectively. These structures reveal that conjugative pili are assemblies of stoichiometric protein-phospholipid units. We further demonstrate that each pilus type binds preferentially to particular phospholipids. These structures provide the molecular basis for F pilus assembly and also shed light on the remarkable properties of conjugative pili in bacterial secretion and phage infection. | ||
| - | + | Structure of the Bacterial Sex F Pilus Reveals an Assembly of a Stoichiometric Protein-Phospholipid Complex.,Costa TR, Ilangovan A, Ukleja M, Redzej A, Santini JM, Smith TK, Egelman EH, Waksman G Cell. 2016 Sep 8;166(6):1436-1444.e10. doi: 10.1016/j.cell.2016.08.025. PMID:27610568<ref>PMID:27610568</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 5leg" style="background-color:#fffaf0;"></div> | |
| - | [[Category: | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Salmonella enterica subsp. enterica serovar typhi]] | ||
| + | [[Category: Costa, T R.D]] | ||
| + | [[Category: Egelman, E H]] | ||
| + | [[Category: Ilangovan, I]] | ||
| + | [[Category: Redzej, A]] | ||
| + | [[Category: Santini, J M]] | ||
| + | [[Category: Smith, T K]] | ||
| + | [[Category: Ukleja, M]] | ||
| + | [[Category: Waksman, G]] | ||
| + | [[Category: F-pilus conjugation type iv secretion system phospholipid]] | ||
| + | [[Category: Protein fibril]] | ||
Revision as of 16:58, 3 October 2016
Warning: this is a large structure, and loading might take a long time or not happen at all.
Structure of the bacterial sex F pilus (pED208)
| |||||||||||
