1pn4
From Proteopedia
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|PDB= 1pn4 |SIZE=350|CAPTION= <scene name='initialview01'>1pn4</scene>, resolution 2.35Å | |PDB= 1pn4 |SIZE=350|CAPTION= <scene name='initialview01'>1pn4</scene>, resolution 2.35Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=HDC:3R-HYDROXYDECANOYL-COENZYME+A'>HDC</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= FOX2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5482 Candida tropicalis]) | |GENE= FOX2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5482 Candida tropicalis]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1pn2|1PN2]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pn4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pn4 OCA], [http://www.ebi.ac.uk/pdbsum/1pn4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1pn4 RCSB]</span> | ||
}} | }} | ||
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[[Category: Hiltunen, J K.]] | [[Category: Hiltunen, J K.]] | ||
[[Category: Koski, M K.]] | [[Category: Koski, M K.]] | ||
| - | [[Category: EDO]] | ||
| - | [[Category: HDC]] | ||
[[Category: enzyme-product complex]] | [[Category: enzyme-product complex]] | ||
[[Category: hot-dog fold]] | [[Category: hot-dog fold]] | ||
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[[Category: oxyanion hole]] | [[Category: oxyanion hole]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:01:51 2008'' |
Revision as of 20:01, 30 March 2008
| |||||||
| , resolution 2.35Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | FOX2 (Candida tropicalis) | ||||||
| Related: | 1PN2
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of 2-enoyl-CoA hydratase 2 domain of Candida tropicalis multifunctional enzyme type 2 complexed with (3R)-hydroxydecanoyl-CoA.
Overview
2-Enoyl-CoA hydratase 2, a part from multifunctional enzyme type 2, hydrates trans-2-enoyl-CoA to 3-hydroxyacyl-CoA in the (3R)-hydroxy-dependent route of peroxisomal beta-oxidation of fatty acids. Unliganded and (3R)-hydroxydecanoyl coenzyme A-complexed crystal structures of 2-enoyl-CoA hydratase 2 from Candida tropicalis multifunctional enzyme type 2 were solved to 1.95- and 2.35-A resolution, respectively. 2-Enoyl-CoA hydratase 2 is a dimeric, alpha+beta protein with a novel quaternary structure. The overall structure of the two-domain subunit of eukaryotic 2-enoyl-CoA hydratase 2 resembles the homodimeric, hot dog fold structures of prokaryotic (R)-specific 2-enoyl-CoA hydratase and beta-hydroxydecanoyl thiol ester dehydrase. Importantly, though, the eukaryotic hydratase 2 has a complete hot dog fold only in its C-domain, whereas the N-domain lacks a long central alpha-helix, thus creating space for bulkier substrates in the binding pocket and explaining the observed difference in substrate preference between eukaryotic and prokaryotic enzymes. Although the N- and C-domains have an identity of <10% at the amino acid level, they share a 50% identity at the nucleotide level and fold similarly. We suggest that a subunit of 2-enoyl-CoA hydratase 2 has evolved via a gene duplication with the concomitant loss of one catalytic site. The hydrogen bonding network of the active site of 2-enoyl-CoA hydratase 2 resembles the active site geometry of mitochondrial (S)-specific 2-enoyl-CoA hydratase 1, although in a mirror image fashion. This arrangement allows the reaction to occur by similar mechanism, supported by mutagenesis and mechanistic studies, although via reciprocal stereochemistry.
About this Structure
1PN4 is a Single protein structure of sequence from Candida tropicalis. Full crystallographic information is available from OCA.
Reference
A two-domain structure of one subunit explains unique features of eukaryotic hydratase 2., Koski MK, Haapalainen AM, Hiltunen JK, Glumoff T, J Biol Chem. 2004 Jun 4;279(23):24666-72. Epub 2004 Mar 29. PMID:15051722
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