1pzt
From Proteopedia
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|PDB= 1pzt |SIZE=350|CAPTION= <scene name='initialview01'>1pzt</scene>, resolution 1.92Å | |PDB= 1pzt |SIZE=350|CAPTION= <scene name='initialview01'>1pzt</scene>, resolution 1.92Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | + | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/N-acetyllactosamine_synthase N-acetyllactosamine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.90 2.4.1.90] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetyllactosamine_synthase N-acetyllactosamine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.90 2.4.1.90] </span> |
|GENE= B4GALT1, GGTB2 OR GALT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 Bos taurus]) | |GENE= B4GALT1, GGTB2 OR GALT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 Bos taurus]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1fgx|1FGX]], [[1o0r|1O0R]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pzt OCA], [http://www.ebi.ac.uk/pdbsum/1pzt PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1pzt RCSB]</span> | ||
}} | }} | ||
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[[Category: Ramakrishnan, B.]] | [[Category: Ramakrishnan, B.]] | ||
[[Category: Ramasamy, V.]] | [[Category: Ramasamy, V.]] | ||
| - | [[Category: | + | [[Category: beta1,4-galactosyltransferase-i tryptophan mutant]] |
| - | + | ||
| - | + | ||
[[Category: catalytic mechanism]] | [[Category: catalytic mechanism]] | ||
[[Category: flexible loop conformation]] | [[Category: flexible loop conformation]] | ||
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[[Category: substrate binding]] | [[Category: substrate binding]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:06:51 2008'' |
Revision as of 20:06, 30 March 2008
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| , resolution 1.92Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | B4GALT1, GGTB2 OR GALT (Bos taurus) | ||||||
| Activity: | N-acetyllactosamine synthase, with EC number 2.4.1.90 | ||||||
| Related: | 1FGX, 1O0R
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF W314A-BETA-1,4-GALACTOSYLTRANSFERASE (B4GAL-T1) CATALYTIC DOMAIN WITHOUT SUBSTRATE
Overview
beta1,4-Galactosyltransferase-I (beta4Gal-T1) undergoes critical conformational changes upon substrate binding from an open conformation (conf-I) to the closed conformation (conf-II). This change involves two flexible loops: the small (residues 313-316) and the long loop (residues 345-365). Upon substrate binding, Trp314 in the small flexible loop moves towards the catalytic pocket and interacts with the donor and the acceptor substrates. For a better understanding of the role played by Trp314 in the conformational changes of beta4Gal-T1, we mutated it to Ala and carried out substrate-binding, proteolytic and crystallographic studies. The W314A mutation reduces the enzymatic activity, binding to substrates and to the modifier protein, alpha-lactalbumin (LA), by over 99%. The limited proteolysis with Glu-C or Lys-C proteases shows differences in the rate of cleavage of the long loop of the wild-type and mutant W314A, indicating conformational differences in the region between the two proteins. Without substrate, the mutant crystallizes in a conformation (conf-I') (1.9A resolution crystal structure), that is not identical with, but close to an open conformation (conf-I), whereas its complex with the substrates and alpha-lactalbumin, crystallizes in a conformation (2.3A resolution crystal structure) that is identical with the closed conformation (conf-II). This study shows the crucial role Trp314 plays in the conformational state of the long loop, in the binding of substrates and in the catalytic mechanism of the enzyme.
About this Structure
1PZT is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
The role of tryptophan 314 in the conformational changes of beta1,4-galactosyltransferase-I., Ramasamy V, Ramakrishnan B, Boeggeman E, Qasba PK, J Mol Biol. 2003 Aug 29;331(5):1065-76. PMID:12927542
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