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470d
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| - | + | ==CRYSTAL STRUCTURE AND IMPROVED ANTISENSE PROPERTIES OF 2'-O-(2-METHOXYETHYL)-RNA== | |
| - | + | <StructureSection load='470d' size='340' side='right' caption='[[470d]], [[Resolution|resolution]] 1.95Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | | | + | <table><tr><td colspan='2'>[[470d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=470D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=470D FirstGlance]. <br> |
| - | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |
| - | | | + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=A44:2-O-METHYOXYETHYL-ADENOSINE+5-MONOPHOSPHATE'>A44</scene>, <scene name='pdbligand=C43:2-O-METHYOXYETHYL-CYTIDINE-5-MONOPHOSPHATE'>C43</scene>, <scene name='pdbligand=G48:2-O-METHYOXYETHYL-GUANOSINE-5-MONOPHOSPHATE'>G48</scene>, <scene name='pdbligand=U36:2-O-METHYOXYETHYL-URIDINE-5-MONOPHOSPHATE'>U36</scene></td></tr> |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=470d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=470d OCA], [http://pdbe.org/470d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=470d RCSB], [http://www.ebi.ac.uk/pdbsum/470d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=470d ProSAT]</span></td></tr> | |
| - | + | </table> | |
| - | ''' | + | <div style="background-color:#fffaf0;"> |
| - | + | == Publication Abstract from PubMed == | |
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2'-O-(2-Methoxyethyl)-RNA (MOE-RNA) is a nucleic acid analog with promising features for antisense applications. Compared with phosphorothioate DNA (PS-DNA), the MOE modification offers improved nuclease resistance, enhanced RNA affinity, improved cellular uptake and intestinal absorption, reduced toxicity and immune stimulation. The crystal structure of a fully modified MOE-RNA dodecamer duplex (CGCGAAUUCGCG) was determined at 1.7 A resolution. In the majority of the MOE substituents, the torsion angle around the ethylene alkyl chain assumes a gauche conformation. The conformational preorganization of the MOE groups is consistent with the improved RNA affinity and the extensive hydration of the substituents could play a role in the improved cellular uptake of MOE-RNA. A specific hydration pattern that bridges substituent and phosphate oxygen atoms in the minor groove of MOE-RNA may explain its high nuclease resistance. | 2'-O-(2-Methoxyethyl)-RNA (MOE-RNA) is a nucleic acid analog with promising features for antisense applications. Compared with phosphorothioate DNA (PS-DNA), the MOE modification offers improved nuclease resistance, enhanced RNA affinity, improved cellular uptake and intestinal absorption, reduced toxicity and immune stimulation. The crystal structure of a fully modified MOE-RNA dodecamer duplex (CGCGAAUUCGCG) was determined at 1.7 A resolution. In the majority of the MOE substituents, the torsion angle around the ethylene alkyl chain assumes a gauche conformation. The conformational preorganization of the MOE groups is consistent with the improved RNA affinity and the extensive hydration of the substituents could play a role in the improved cellular uptake of MOE-RNA. A specific hydration pattern that bridges substituent and phosphate oxygen atoms in the minor groove of MOE-RNA may explain its high nuclease resistance. | ||
| - | + | Crystal structure and improved antisense properties of 2'-O-(2-methoxyethyl)-RNA.,Teplova M, Minasov G, Tereshko V, Inamati GB, Cook PD, Manoharan M, Egli M Nat Struct Biol. 1999 Jun;6(6):535-9. PMID:10360355<ref>PMID:10360355</ref> | |
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| - | Crystal structure and improved antisense properties of 2'-O-(2-methoxyethyl)-RNA., Teplova M, Minasov G, Tereshko V, Inamati GB, Cook PD, Manoharan M, Egli M | + | |
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| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 470d" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Cook, P D]] | ||
| + | [[Category: Egli, M]] | ||
| + | [[Category: Inamati, G]] | ||
| + | [[Category: Minasov, G]] | ||
| + | [[Category: Teplova, M]] | ||
| + | [[Category: Tereshko, V]] | ||
| + | [[Category: Ribonucleic acid]] | ||
| + | [[Category: Rna]] | ||
| + | [[Category: Rna dodecamer duplex]] | ||
Current revision
CRYSTAL STRUCTURE AND IMPROVED ANTISENSE PROPERTIES OF 2'-O-(2-METHOXYETHYL)-RNA
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