This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
470d
From Proteopedia
(Difference between revisions)
| (5 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | [[Image:470d.gif|left|200px]] | ||
| - | + | ==CRYSTAL STRUCTURE AND IMPROVED ANTISENSE PROPERTIES OF 2'-O-(2-METHOXYETHYL)-RNA== | |
| - | + | <StructureSection load='470d' size='340' side='right' caption='[[470d]], [[Resolution|resolution]] 1.95Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[470d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=470D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=470D FirstGlance]. <br> | |
| - | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |
| - | --> | + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=A44:2-O-METHYOXYETHYL-ADENOSINE+5-MONOPHOSPHATE'>A44</scene>, <scene name='pdbligand=C43:2-O-METHYOXYETHYL-CYTIDINE-5-MONOPHOSPHATE'>C43</scene>, <scene name='pdbligand=G48:2-O-METHYOXYETHYL-GUANOSINE-5-MONOPHOSPHATE'>G48</scene>, <scene name='pdbligand=U36:2-O-METHYOXYETHYL-URIDINE-5-MONOPHOSPHATE'>U36</scene></td></tr> |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=470d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=470d OCA], [http://pdbe.org/470d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=470d RCSB], [http://www.ebi.ac.uk/pdbsum/470d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=470d ProSAT]</span></td></tr> | |
| - | + | </table> | |
| - | ''' | + | <div style="background-color:#fffaf0;"> |
| - | + | == Publication Abstract from PubMed == | |
| - | + | ||
| - | == | + | |
2'-O-(2-Methoxyethyl)-RNA (MOE-RNA) is a nucleic acid analog with promising features for antisense applications. Compared with phosphorothioate DNA (PS-DNA), the MOE modification offers improved nuclease resistance, enhanced RNA affinity, improved cellular uptake and intestinal absorption, reduced toxicity and immune stimulation. The crystal structure of a fully modified MOE-RNA dodecamer duplex (CGCGAAUUCGCG) was determined at 1.7 A resolution. In the majority of the MOE substituents, the torsion angle around the ethylene alkyl chain assumes a gauche conformation. The conformational preorganization of the MOE groups is consistent with the improved RNA affinity and the extensive hydration of the substituents could play a role in the improved cellular uptake of MOE-RNA. A specific hydration pattern that bridges substituent and phosphate oxygen atoms in the minor groove of MOE-RNA may explain its high nuclease resistance. | 2'-O-(2-Methoxyethyl)-RNA (MOE-RNA) is a nucleic acid analog with promising features for antisense applications. Compared with phosphorothioate DNA (PS-DNA), the MOE modification offers improved nuclease resistance, enhanced RNA affinity, improved cellular uptake and intestinal absorption, reduced toxicity and immune stimulation. The crystal structure of a fully modified MOE-RNA dodecamer duplex (CGCGAAUUCGCG) was determined at 1.7 A resolution. In the majority of the MOE substituents, the torsion angle around the ethylene alkyl chain assumes a gauche conformation. The conformational preorganization of the MOE groups is consistent with the improved RNA affinity and the extensive hydration of the substituents could play a role in the improved cellular uptake of MOE-RNA. A specific hydration pattern that bridges substituent and phosphate oxygen atoms in the minor groove of MOE-RNA may explain its high nuclease resistance. | ||
| - | + | Crystal structure and improved antisense properties of 2'-O-(2-methoxyethyl)-RNA.,Teplova M, Minasov G, Tereshko V, Inamati GB, Cook PD, Manoharan M, Egli M Nat Struct Biol. 1999 Jun;6(6):535-9. PMID:10360355<ref>PMID:10360355</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | [[Category: Cook, P D | + | <div class="pdbe-citations 470d" style="background-color:#fffaf0;"></div> |
| - | [[Category: Egli, M | + | == References == |
| - | [[Category: Inamati, G | + | <references/> |
| - | [[Category: Minasov, G | + | __TOC__ |
| - | [[Category: Teplova, M | + | </StructureSection> |
| - | [[Category: Tereshko, V | + | [[Category: Cook, P D]] |
| + | [[Category: Egli, M]] | ||
| + | [[Category: Inamati, G]] | ||
| + | [[Category: Minasov, G]] | ||
| + | [[Category: Teplova, M]] | ||
| + | [[Category: Tereshko, V]] | ||
[[Category: Ribonucleic acid]] | [[Category: Ribonucleic acid]] | ||
| + | [[Category: Rna]] | ||
[[Category: Rna dodecamer duplex]] | [[Category: Rna dodecamer duplex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:19:28 2008'' | ||
Current revision
CRYSTAL STRUCTURE AND IMPROVED ANTISENSE PROPERTIES OF 2'-O-(2-METHOXYETHYL)-RNA
| |||||||||||
