1rio
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1rio |SIZE=350|CAPTION= <scene name='initialview01'>1rio</scene>, resolution 2.30Å | |PDB= 1rio |SIZE=350|CAPTION= <scene name='initialview01'>1rio</scene>, resolution 2.30Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> | + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= CI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Enterobacteria phage lambda]) | |GENE= CI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Enterobacteria phage lambda]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1ku7|1KU7]], [[1lmb|1LMB]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rio OCA], [http://www.ebi.ac.uk/pdbsum/1rio PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rio RCSB]</span> | ||
}} | }} | ||
| Line 28: | Line 31: | ||
[[Category: Nickels, B E.]] | [[Category: Nickels, B E.]] | ||
[[Category: Sun, L.]] | [[Category: Sun, L.]] | ||
| - | [[Category: CA]] | ||
| - | [[Category: MPD]] | ||
[[Category: helix-turn-helix]] | [[Category: helix-turn-helix]] | ||
[[Category: transcription activation]] | [[Category: transcription activation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:28:40 2008'' |
Revision as of 20:28, 30 March 2008
| |||||||
| , resolution 2.30Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , , , | ||||||
| Gene: | CI (Enterobacteria phage lambda) | ||||||
| Related: | 1KU7, 1LMB
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of bacteriophage lambda cI-NTD in complex with sigma-region4 of Thermus aquaticus bound to DNA
Overview
The cI protein of bacteriophage lambda (lambdacI) activates transcription by binding a DNA operator just upstream of the promoter and interacting with the RNA polymerase sigma subunit domain 4 (sigma(4)). We determined the crystal structure of the lambdacI/sigma(4)/DNA ternary complex at 2.3 A resolution. There are no conformational changes in either protein, which interact through an extremely small interface involving at most 6 amino acid residues. The interactions of the two proteins stabilize the binding of each protein to the DNA. The results provide insight into how activators can operate through a simple cooperative binding mechanism but affect different steps of the transcription initiation process.
About this Structure
1RIO is a Protein complex structure of sequences from Enterobacteria phage lambda and Thermus aquaticus. Full crystallographic information is available from OCA.
Reference
Structure of a ternary transcription activation complex., Jain D, Nickels BE, Sun L, Hochschild A, Darst SA, Mol Cell. 2004 Jan 16;13(1):45-53. PMID:14731393
Page seeded by OCA on Sun Mar 30 23:28:40 2008
