1ryh
From Proteopedia
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|PDB= 1ryh |SIZE=350|CAPTION= <scene name='initialview01'>1ryh</scene>, resolution 1.75Å | |PDB= 1ryh |SIZE=350|CAPTION= <scene name='initialview01'>1ryh</scene>, resolution 1.75Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Small_monomeric_GTPase Small monomeric GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.2 3.6.5.2] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Small_monomeric_GTPase Small monomeric GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.2 3.6.5.2] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1ryf|1RYF]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ryh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ryh OCA], [http://www.ebi.ac.uk/pdbsum/1ryh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ryh RCSB]</span> | ||
}} | }} | ||
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[[Category: Ahmadian, M R.]] | [[Category: Ahmadian, M R.]] | ||
[[Category: Fiegen, D.]] | [[Category: Fiegen, D.]] | ||
| - | [[Category: GNP]] | ||
| - | [[Category: MG]] | ||
[[Category: gtp binding]] | [[Category: gtp binding]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:34:45 2008'' |
Revision as of 20:34, 30 March 2008
| |||||||
| , resolution 1.75Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Activity: | Small monomeric GTPase, with EC number 3.6.5.2 | ||||||
| Related: | 1RYF
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Alternative Splicing of Rac1 Generates Rac1b, a Self-activating GTPase
Overview
Rac1b was recently identified in malignant colorectal tumors as an alternative splice variant of Rac1 containing a 19-amino acid insertion next to the switch II region. The structures of Rac1b in the GDP- and the GppNHp-bound forms, determined at a resolution of 1.75 A, reveal that the insertion induces an open switch I conformation and a highly mobile switch II. As a consequence, Rac1b has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. Interestingly, Rac1b is able to bind the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction. The presented study provides insights into the structural and biochemical mechanism of a self-activating GTPase.
About this Structure
1RYH is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Alternative splicing of Rac1 generates Rac1b, a self-activating GTPase., Fiegen D, Haeusler LC, Blumenstein L, Herbrand U, Dvorsky R, Vetter IR, Ahmadian MR, J Biol Chem. 2004 Feb 6;279(6):4743-9. Epub 2003 Nov 18. PMID:14625275
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