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1s6r

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|PDB= 1s6r |SIZE=350|CAPTION= <scene name='initialview01'>1s6r</scene>, resolution 2.24&Aring;
|PDB= 1s6r |SIZE=350|CAPTION= <scene name='initialview01'>1s6r</scene>, resolution 2.24&Aring;
|SITE=
|SITE=
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|LIGAND= <scene name='pdbligand=IAP:4-IODO-ACETAMIDO PHENYLBORONIC ACID'>IAP</scene>
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|LIGAND= <scene name='pdbligand=IAP:4-IODO-ACETAMIDO+PHENYLBORONIC+ACID'>IAP</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Hydrolase Hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6. 3.5.2.6.]
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrolase Hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6. 3.5.2.6.] </span>
|GENE=
|GENE=
 +
|DOMAIN=
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|RELATEDENTRY=[[3bls|3BLS]], [[1kds|1KDS]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s6r OCA], [http://www.ebi.ac.uk/pdbsum/1s6r PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1s6r RCSB]</span>
}}
}}
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[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Wouters, J.]]
[[Category: Wouters, J.]]
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[[Category: IAP]]
 
[[Category: hydrolase]]
[[Category: hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:01:11 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:38:11 2008''

Revision as of 20:38, 30 March 2008


PDB ID 1s6r

Drag the structure with the mouse to rotate
, resolution 2.24Å
Ligands:
Activity: Hydrolase, with EC number 3.5.2.6.
Related: 3BLS, 1KDS


Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



908R CLASS C BETA-LACTAMASE BOUND TO IODO-ACETAMIDO-PHENYL BORONIC ACID


Overview

The structures of the class C beta-lactamase from Enterobacter cloacae 908R alone and in complex with a boronic acid transition-state analogue were determined by X-ray crystallography at 2.1 and 2.3 A, respectively. The structure of the enzyme resembles those of other class C beta-lactamases. The structure of the complex with the transition-state analogue, iodo-acetamido-phenyl boronic acid, shows that the inhibitor is covalently bound to the active-site serine (Ser64). Binding of the inhibitor within the active site is compared with previously determined structures of complexes with other class C enzymes. The structure of the boronic acid adduct indicates ways to improve the affinity of this class of inhibitors. This structure of 908R class C beta-lactamase in complex with a transition-state analogue provides further insights into the mechanism of action of these hydrolases.

About this Structure

1S6R is a Single protein structure of sequence from Enterobacter cloacae. Full crystallographic information is available from OCA.

Reference

Crystal structure of Enterobacter cloacae 908R class C beta-lactamase bound to iodo-acetamido-phenyl boronic acid, a transition-state analogue., Wouters J, Fonze E, Vermeire M, Frere JM, Charlier P, Cell Mol Life Sci. 2003 Aug;60(8):1764-73. PMID:14521155

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