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4cgb

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Revision as of 18:34, 1 February 2017

Crystal structure of the trimerization domain of EML2

Structural highlights

4cgb is a 6 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	4cgc
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[EMAL2_HUMAN] Tubulin binding protein that inhibits microtubule nucleation and growth, resulting in shorter microtubules.^[1]

Publication Abstract from PubMed

Proteins of the echinoderm microtubule associated protein-like (EML) family contribute to formation of the mitotic spindle and interphase microtubule (MT) network. EML1-4 consist of WD40 repeats and an N-terminal region containing a putative coiled-coil. Recurrent gene rearrangements in non-small cell lung cancer (NSCLC) fuse EML4 to anaplastic lymphoma kinase (ALK) causing expression of several oncogenic fusion variants. The fusions have constitutive ALK activity due to self-association through the EML4 coiled-coil. We have determined crystal structures of the coiled-coils from EML2 and EML4, which describe the structural basis of both EML self-association and oncogenic EML4-ALK activation. The structures reveal a trimeric oligomerization state directed by a conserved pattern of hydrophobic residues and salt bridges. We show that the trimerization domain (TD) of EML1 is necessary and sufficient for self-association. The TD is also essential for MT binding, however this property requires an adjacent basic region. These observations prompted us to investigate MT association of EML4-ALK and EML1-ABL1 fusions in which variable portions of the EML component are present. Uniquely, EML4-ALK variant 3, which includes the TD and basic region of EML4 but none of the WD40 repeats, was localized to MTs, both when expressed recombinantly and in a patient-derived NSCLC cell line (H2228). This raises the question of whether the mislocalization of ALK activity to MTs might influence downstream signalling and malignant properties of cells. Furthermore, the structure of EML4 TD may enable the development of protein-protein interaction inhibitors targeting the trimerization interface, providing a possible avenue towards therapeutic intervention in EML4-ALK NSCLC.

Microtubule association of EML proteins and the EML4-ALK variant 3 oncoprotein require an N-terminal trimerization domain.,Richards MW, O'Regan L, Roth D, Montgomery JM, Straube A, Fry AM, Bayliss R Biochem J. 2015 Mar 5. PMID:25740311^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Eichenmuller B, Everley P, Palange J, Lepley D, Suprenant KA. The human EMAP-like protein-70 (ELP70) is a microtubule destabilizer that localizes to the mitotic apparatus. J Biol Chem. 2002 Jan 11;277(2):1301-9. Epub 2001 Nov 2. PMID:11694528 doi:http://dx.doi.org/10.1074/jbc.M106628200
↑ Richards MW, O'Regan L, Roth D, Montgomery JM, Straube A, Fry AM, Bayliss R. Microtubule association of EML proteins and the EML4-ALK variant 3 oncoprotein require an N-terminal trimerization domain. Biochem J. 2015 Mar 5. PMID:25740311 doi:http://dx.doi.org/10.1042/BJ20150039

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4cgb"

Categories: Bayliss, R | Richards, M W | Cell cycle | Coiled-coil

@@ Line 1: / Line 1: @@
 ==Crystal structure of the trimerization domain of EML2==
 <StructureSection load='4cgb' size='340' side='right' caption='[[4cgb]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4cgc|4cgc]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cgb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cgb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cgb RCSB], [http://www.ebi.ac.uk/pdbsum/4cgb PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cgb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cgb OCA], [http://pdbe.org/4cgb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cgb RCSB], [http://www.ebi.ac.uk/pdbsum/4cgb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4cgb ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4cgb" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4cgb

From Proteopedia

Revision as of 18:34, 1 February 2017

Crystal structure of the trimerization domain of EML2

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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