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1t1a
From Proteopedia
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|PDB= 1t1a |SIZE=350|CAPTION= <scene name='initialview01'>1t1a</scene>, resolution 1.60Å | |PDB= 1t1a |SIZE=350|CAPTION= <scene name='initialview01'>1t1a</scene>, resolution 1.60Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=HC4:4'-HYDROXYCINNAMIC ACID'>HC4</scene> | + | |LIGAND= <scene name='pdbligand=HC4:4'-HYDROXYCINNAMIC+ACID'>HC4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= PYP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1053 Halorhodospira halophila]) | |GENE= PYP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1053 Halorhodospira halophila]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1t18|1T18]], [[1t19|1T19]], [[1t1b|1T1B]], [[1t1c|1T1C]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t1a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t1a OCA], [http://www.ebi.ac.uk/pdbsum/1t1a PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1t1a RCSB]</span> | ||
}} | }} | ||
| Line 28: | Line 31: | ||
[[Category: Schmidt, M.]] | [[Category: Schmidt, M.]] | ||
[[Category: Srajer, V.]] | [[Category: Srajer, V.]] | ||
| - | [[Category: HC4]] | ||
[[Category: pas domain]] | [[Category: pas domain]] | ||
[[Category: photoactive yellow protein]] | [[Category: photoactive yellow protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:49:49 2008'' |
Revision as of 20:49, 30 March 2008
| |||||||
| , resolution 1.60Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | PYP (Halorhodospira halophila) | ||||||
| Related: | 1T18, 1T19, 1T1B, 1T1C
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Late intermediate IL1 from time-resolved crystallography of the E46Q mutant of PYP
Overview
In the bacterial photoreceptor photoactive yellow protein (PYP), absorption of blue light by its chromophore leads to a conformational change in the protein associated with differential signaling activity, as it executes a reversible photocycle. Time-resolved Laue crystallography allows structural snapshots (as short as 150 ps) of high crystallographic resolution (approximately 1.6 A) to be taken of a protein as it functions. Here, we analyze by singular value decomposition a comprehensive time-resolved crystallographic data set of the E46Q mutant of PYP throughout the photocycle spanning 10 ns-100 ms. We identify and refine the structures of five distinct intermediates and provide a plausible chemical kinetic mechanism for their inter conversion. A clear structural progression is visible in these intermediates, in which a signal generated at the chromophore propagates through a distinct structural pathway of conserved residues and results in structural changes near the N terminus, over 20 A distant from the chromophore.
About this Structure
1T1A is a Single protein structure of sequence from Halorhodospira halophila. Full crystallographic information is available from OCA.
Reference
A structural pathway for signaling in the E46Q mutant of photoactive yellow protein., Rajagopal S, Anderson S, Srajer V, Schmidt M, Pahl R, Moffat K, Structure. 2005 Jan;13(1):55-63. PMID:15642261
Page seeded by OCA on Sun Mar 30 23:49:49 2008
