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4x8k

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Revision as of 14:25, 29 March 2017

Mycobacterium tuberculosis RbpA-SID in complex with SigmaA domain 2

Structural highlights

4x8k is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SIGA_MYCTO] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.[HAMAP-Rule:MF_00963] [RBPA_MYCTO] Binds to RNA polymerase (RNAP), stimulating transcription from principal, but not alternative sigma factor promoters.

Publication Abstract from PubMed

Gene expression is highly regulated at the step of transcription initiation, and transcription activators play a critical role in this process. RbpA, an actinobacterial transcription activator that is essential in Mycobacterium tuberculosis (Mtb), binds selectively to group 1 and certain group 2 sigma-factors. To delineate the molecular mechanism of RbpA, we show that the Mtb RbpA sigma-interacting domain (SID) and basic linker are sufficient for transcription activation. We also present the crystal structure of the Mtb RbpA-SID in complex with domain 2 of the housekeeping sigma-factor, sigma(A). The structure explains the basis of sigma-selectivity by RbpA, showing that RbpA interacts with conserved regions of sigma(A) as well as the nonconserved region (NCR), which is present only in housekeeping sigma-factors. Thus, the structure is the first, to our knowledge, to show a protein interacting with the NCR of a sigma-factor. We confirm the basis of selectivity and the observed interactions using mutagenesis and functional studies. In addition, the structure allows for a model of the RbpA-SID in the context of a transcription initiation complex. Unexpectedly, the structural modeling suggests that RbpA contacts the promoter DNA, and we present in vivo and in vitro studies supporting this finding. Our combined data lead to a better understanding of the mechanism of RbpA function as a transcription activator.

Structural, functional, and genetic analyses of the actinobacterial transcription factor RbpA.,Hubin EA, Tabib-Salazar A, Humphrey LJ, Flack JE, Olinares PD, Darst SA, Campbell EA, Paget MS Proc Natl Acad Sci U S A. 2015 Jun 9;112(23):7171-6. doi:, 10.1073/pnas.1504942112. Epub 2015 May 26. PMID:26040003^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hubin EA, Tabib-Salazar A, Humphrey LJ, Flack JE, Olinares PD, Darst SA, Campbell EA, Paget MS. Structural, functional, and genetic analyses of the actinobacterial transcription factor RbpA. Proc Natl Acad Sci U S A. 2015 Jun 9;112(23):7171-6. doi:, 10.1073/pnas.1504942112. Epub 2015 May 26. PMID:26040003 doi:http://dx.doi.org/10.1073/pnas.1504942112

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4x8k"

@@ Line 1: / Line 1: @@
 ==Mycobacterium tuberculosis RbpA-SID in complex with SigmaA domain 2==
 <StructureSection load='4x8k' size='340' side='right' caption='[[4x8k]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
@@ Line 4: / Line 5: @@
 <table><tr><td colspan='2'>[[4x8k]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X8K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4X8K FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x8k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4x8k RCSB], [http://www.ebi.ac.uk/pdbsum/4x8k PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x8k OCA], [http://pdbe.org/4x8k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4x8k RCSB], [http://www.ebi.ac.uk/pdbsum/4x8k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4x8k ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 16: / Line 17: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4x8k" style="background-color:#fffaf0;"></div>
 ==See Also==

4x8k

From Proteopedia

Revision as of 14:25, 29 March 2017

Mycobacterium tuberculosis RbpA-SID in complex with SigmaA domain 2

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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