1u3e
From Proteopedia
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|PDB= 1u3e |SIZE=350|CAPTION= <scene name='initialview01'>1u3e</scene>, resolution 2.92Å | |PDB= 1u3e |SIZE=350|CAPTION= <scene name='initialview01'>1u3e</scene>, resolution 2.92Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SR:STRONTIUM+ION'>SR</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene> |
|ACTIVITY= | |ACTIVITY= | ||
| - | |GENE= bacteriophage SP01 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= | + | |GENE= bacteriophage SP01 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10685 Bacillus phage SPO1]) |
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1i3j|1I3J]], [[1a73|1A73]], [[1fsj|1FSJ]], [[1pt3|1PT3]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u3e OCA], [http://www.ebi.ac.uk/pdbsum/1u3e PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1u3e RCSB]</span> | ||
}} | }} | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1U3E is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | + | 1U3E is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_phage_spo1 Bacillus phage spo1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U3E OCA]. |
==Reference== | ==Reference== | ||
DNA binding and cleavage by the HNH homing endonuclease I-HmuI., Shen BW, Landthaler M, Shub DA, Stoddard BL, J Mol Biol. 2004 Sep 3;342(1):43-56. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15313606 15313606] | DNA binding and cleavage by the HNH homing endonuclease I-HmuI., Shen BW, Landthaler M, Shub DA, Stoddard BL, J Mol Biol. 2004 Sep 3;342(1):43-56. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15313606 15313606] | ||
| - | [[Category: | + | [[Category: Bacillus phage spo1]] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Landthaler, M.]] | [[Category: Landthaler, M.]] | ||
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[[Category: Shub, D A.]] | [[Category: Shub, D A.]] | ||
[[Category: Stoddard, B L.]] | [[Category: Stoddard, B L.]] | ||
| - | [[Category: EDO]] | ||
| - | [[Category: MN]] | ||
| - | [[Category: SR]] | ||
| - | [[Category: TRS]] | ||
[[Category: helix-turn-helix dna binding domain]] | [[Category: helix-turn-helix dna binding domain]] | ||
[[Category: hnh catalytic motif]] | [[Category: hnh catalytic motif]] | ||
| - | [[Category: protein-dna complex]] | + | [[Category: protein-dna complex,]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:04:55 2008'' |
Revision as of 21:04, 30 March 2008
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| , resolution 2.92Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , , , , | ||||||
| Gene: | bacteriophage SP01 (Bacillus phage SPO1) | ||||||
| Related: | 1I3J, 1A73, 1FSJ, 1PT3
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
DNA binding and cleavage by the HNH homing endonuclease I-HmuI
Overview
The structure of I-HmuI, which represents the last family of homing endonucleases without a defining crystallographic structure, has been determined in complex with its DNA target. A series of diverse protein structural domains and motifs, contacting sequential stretches of nucleotide bases, are distributed along the DNA target. I-HmuI contains an N-terminal domain with a DNA-binding surface found in the I-PpoI homing endonuclease and an associated HNH/N active site found in the bacterial colicins, and a C-terminal DNA-binding domain previously observed in the I-TevI homing endonuclease. The combination and exchange of these features between protein families indicates that the genetic mobility associated with homing endonucleases extends to the level of independent structural domains. I-HmuI provides an unambiguous structural connection between the His-Cys box endonucleases and the bacterial colicins, supporting the hypothesis that these enzymes diverged from a common ancestral nuclease.
About this Structure
1U3E is a Single protein structure of sequence from Bacillus phage spo1. Full crystallographic information is available from OCA.
Reference
DNA binding and cleavage by the HNH homing endonuclease I-HmuI., Shen BW, Landthaler M, Shub DA, Stoddard BL, J Mol Biol. 2004 Sep 3;342(1):43-56. PMID:15313606
Page seeded by OCA on Mon Mar 31 00:04:55 2008
