DTDP-glucose 4,6-dehydratase

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Revision as of 06:49, 27 April 2017

spinqualitylabelsall models RmlB dimer complex with NAD, TDP-D-glucose and sulfate (PDB code 1ker) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image Function dTDP-glucose 4,6-dehydratase (RmlB) catalyzes the reversible conversion of dTDP-glucose to dTDP-4-dehydro-6-deoxy-D-glucose. RmlB is the second of four enzymes involved in the dTDP-L-rhamnose pathway[1]. RmlB is involved in the pathway LSP O-antigen biosynthesis which is part of the bacterial outer membrane biogenesis[2]. Structural highlights The RmlB structure is divided into 2 domains. The N-terminal NAD-binding domain which contains the Rossmann fold and the C-terminal sugar nucleotide binding domain[3]. . . .

3D structures of RmlB

Updated on 27-April-2017

1kep, 1oc2 – SsRmlB + NAD + TDP-xylose – Streptococcus suis
1ker – SsRmlB + NAD + TDP-glucose
1ket – SsRmlB + NAD + TDP
1keu – StRmlB + NAD + TDP-glucose – Salmonella typhimurium
1kew – StRmlB + NAD + TDP
1g1a – StRmlB + NAD
1bxk – EcRmlB + NAD – Escherichia coli
1r66, 1r6d – RmlB + NAD + TDP derivative – Streptococcus venezuelae
4egb – RmlB + NAD + TDP-sucrose – Bacillus anthracis
5u4q – RmlB + NAD – Klebsiella pneumoniae

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References

1. ↑ Allard ST, Giraud MF, Whitfield C, Messner P, Naismith JH. The purification, crystallization and structural elucidation of dTDP-D-glucose 4,6-dehydratase (RmlB), the second enzyme of the dTDP-L-rhamnose synthesis pathway from Salmonella enterica serovar typhimurium. Acta Crystallogr D Biol Crystallogr. 2000 Feb;56(Pt 2):222-5. PMID:10666612
2. ↑ Allard ST, Giraud MF, Whitfield C, Messner P, Naismith JH. The purification, crystallization and structural elucidation of dTDP-D-glucose 4,6-dehydratase (RmlB), the second enzyme of the dTDP-L-rhamnose synthesis pathway from Salmonella enterica serovar typhimurium. Acta Crystallogr D Biol Crystallogr. 2000 Feb;56(Pt 2):222-5. PMID:10666612
3. ↑ Allard ST, Beis K, Giraud MF, Hegeman AD, Gross JW, Wilmouth RC, Whitfield C, Graninger M, Messner P, Allen AG, Maskell DJ, Naismith JH. Toward a structural understanding of the dehydratase mechanism. Structure. 2002 Jan;10(1):81-92. PMID:11796113