1us7
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1us7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1us7 OCA], [http://www.ebi.ac.uk/pdbsum/1us7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1us7 RCSB]</span> | ||
}} | }} | ||
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[[Category: chaperone]] | [[Category: chaperone]] | ||
[[Category: chaperone co-chaperone regulation]] | [[Category: chaperone co-chaperone regulation]] | ||
| - | [[Category: heat shock]] | + | [[Category: heat shock,]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:14:28 2008'' |
Revision as of 21:14, 30 March 2008
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| , resolution 2.30Å | |||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
COMPLEX OF HSP90 AND P50
Overview
Recruitment of protein kinase clients to the Hsp90 chaperone involves the cochaperone p50(cdc37) acting as a scaffold, binding protein kinases via its N-terminal domain and Hsp90 via its C-terminal region. p50(cdc37) also has a regulatory activity, arresting Hsp90's ATPase cycle during client-protein loading. We have localized the binding site for p50(cdc37) to the N-terminal nucleotide binding domain of Hsp90 and determined the crystal structure of the Hsp90-p50(cdc37) core complex. Dimeric p50(cdc37) binds to surfaces of the Hsp90 N-domain implicated in ATP-dependent N-terminal dimerization and association with the middle segment of the chaperone. This interaction fixes the lid segment in an open conformation, inserts an arginine side chain into the ATP binding pocket to disable catalysis, and prevents trans-activating interaction of the N domains.
About this Structure
1US7 is a Protein complex structure of sequences from Homo sapiens and Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
The Mechanism of Hsp90 regulation by the protein kinase-specific cochaperone p50(cdc37)., Roe SM, Ali MM, Meyer P, Vaughan CK, Panaretou B, Piper PW, Prodromou C, Pearl LH, Cell. 2004 Jan 9;116(1):87-98. PMID:14718169
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