1uth
From Proteopedia
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|PDB= 1uth |SIZE=350|CAPTION= <scene name='initialview01'>1uth</scene>, resolution 2.20Å | |PDB= 1uth |SIZE=350|CAPTION= <scene name='initialview01'>1uth</scene>, resolution 2.20Å | ||
|SITE= <scene name='pdbsite=AC1:Scn+Binding+Site+For+Chain+A'>AC1</scene> | |SITE= <scene name='pdbsite=AC1:Scn+Binding+Site+For+Chain+A'>AC1</scene> | ||
| - | |LIGAND= <scene name='pdbligand=SCN:THIOCYANATE ION'>SCN</scene> | + | |LIGAND= <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uth FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uth OCA], [http://www.ebi.ac.uk/pdbsum/1uth PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1uth RCSB]</span> | ||
}} | }} | ||
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[[Category: Mcsweeney, S.]] | [[Category: Mcsweeney, S.]] | ||
[[Category: Smirnova, I A.]] | [[Category: Smirnova, I A.]] | ||
| - | [[Category: SCN]] | ||
[[Category: lysr]] | [[Category: lysr]] | ||
[[Category: transcriptional regulator]] | [[Category: transcriptional regulator]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:15:01 2008'' |
Revision as of 21:15, 30 March 2008
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| , resolution 2.20Å | |||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
DNTR FROM BURKHOLDERIA SP. STRAIN DNT IN COMPLEX WITH THIOCYANATE
Overview
The transcriptional regulator DntR, a member of the LysR family, is a central element in a prototype bacterial cell-based biosensor for the detection of hazardous contamination of soil and groundwater by dinitrotoluenes. To optimise the sensitivity of the biosensor for such compounds we have chosen a rational design of the inducer-binding cavity based on knowledge of the three-dimensional structure of DntR. We report two crystal structures of DntR with acetate (resolution 2.6 angstroms) and thiocyanate (resolution 2.3 angstroms), respectively, occupying the inducer-binding cavity. These structures allow for the construction of models of DntR in complex with salicylate (Kd approximately or = 4 microM) and 2,4-dinitrotoluene that provide a basis for the design of mutant DntR with enhanced specificity for dinitrotoluenes. In both crystal structures DntR crystallises as a homodimer with a "head-to-tail" arrangement of monomers in the asymmetric unit. Analysis of the crystal structure has allowed the building of a full-length model of DntR in its biologically active homotetrameric form consisting of two "head-to-head" dimers. The implications of this model for the mechanism of transcription regulation by LysR proteins are discussed.
About this Structure
1UTH is a Protein complex structure of sequences from Burkholderia sp.. Full crystallographic information is available from OCA.
Reference
Development of a bacterial biosensor for nitrotoluenes: the crystal structure of the transcriptional regulator DntR., Smirnova IA, Dian C, Leonard GA, McSweeney S, Birse D, Brzezinski P, J Mol Biol. 2004 Jul 9;340(3):405-18. PMID:15210343
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