1vld
From Proteopedia
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|PDB= 1vld |SIZE=350|CAPTION= <scene name='initialview01'>1vld</scene>, resolution 2.35Å | |PDB= 1vld |SIZE=350|CAPTION= <scene name='initialview01'>1vld</scene>, resolution 2.35Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, | + | |LIGAND= <scene name='pdbligand=4MO:MOLYBDENUM(IV)+ION'>4MO</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Pyrogallol_hydroxytransferase Pyrogallol hydroxytransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.97.1.2 1.97.1.2] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyrogallol_hydroxytransferase Pyrogallol hydroxytransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.97.1.2 1.97.1.2] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1ti2|1TI2]], [[1ti4|1TI4]], [[1ti6|1TI6]], [[1vle|1VLE]], [[1vlf|1VLF]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vld FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vld OCA], [http://www.ebi.ac.uk/pdbsum/1vld PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1vld RCSB]</span> | ||
}} | }} | ||
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[[Category: Niessen, H.]] | [[Category: Niessen, H.]] | ||
[[Category: Schink, B.]] | [[Category: Schink, B.]] | ||
| - | [[Category: 4MO]] | ||
| - | [[Category: ACT]] | ||
| - | [[Category: CA]] | ||
| - | [[Category: MGD]] | ||
| - | [[Category: SF4]] | ||
[[Category: 4fe-4s-cluster]] | [[Category: 4fe-4s-cluster]] | ||
[[Category: dmso-reductase family]] | [[Category: dmso-reductase family]] | ||
| Line 39: | Line 37: | ||
[[Category: molybdenum binding enzyme]] | [[Category: molybdenum binding enzyme]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:25:58 2008'' |
Revision as of 21:25, 30 March 2008
| |||||||
| , resolution 2.35Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , | ||||||
| Activity: | Pyrogallol hydroxytransferase, with EC number 1.97.1.2 | ||||||
| Related: | 1TI2, 1TI4, 1TI6, 1VLE, 1VLF
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Pyrogallol-Phloroglucinol Transhydroxylase from Pelobacter acidigallici
Overview
The Mo enzyme transhydroxylase from the anaerobic microorganism Pelobacter acidigallici catalyzes the conversion of pyrogallol to phloroglucinol. Such trihydroxybenzenes and their derivatives represent important building blocks of plant polymers. None of the transferred hydroxyl groups originates from water during transhydroxylation; instead a cosubstrate, such as 1,2,3,5-tetrahydroxybenzene, is used in a reaction without apparent electron transfer. Here, we report on the crystal structure of the enzyme in the reduced Mo(IV) state, which we solved by single anomalous-diffraction technique. It represents the largest structure (1,149 amino acid residues per molecule, 12 independent molecules per unit cell), which has been solved so far by single anomalous-diffraction technique. Tranhydroxylase is a heterodimer, with the active Mo-molybdopterin guanine dinucleotide (MGD)(2) site in the alpha-subunit, and three [4Fe-4S] centers in the beta-subunit. The latter subunit carries a seven-stranded, mainly antiparallel beta-barrel domain. We propose a scheme for the transhydroxylation reaction based on 3D structures of complexes of the enzyme with various polyphenols serving either as substrate or inhibitor.
About this Structure
1VLD is a Protein complex structure of sequences from Pelobacter acidigallici. Full crystallographic information is available from OCA.
Reference
Crystal structure of pyrogallol-phloroglucinol transhydroxylase, an Mo enzyme capable of intermolecular hydroxyl transfer between phenols., Messerschmidt A, Niessen H, Abt D, Einsle O, Schink B, Kroneck PM, Proc Natl Acad Sci U S A. 2004 Aug 10;101(32):11571-6. Epub 2004 Jul 29. PMID:15284442
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