1vz3
From Proteopedia
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|SITE= <scene name='pdbsite=AS1:Engineered+Disulphide+Bridge'>AS1</scene> | |SITE= <scene name='pdbsite=AS1:Engineered+Disulphide+Bridge'>AS1</scene> | ||
|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | |LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | ||
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Prolyl_oligopeptidase Prolyl oligopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.26 3.4.21.26] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Prolyl_oligopeptidase Prolyl oligopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.26 3.4.21.26] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vz3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vz3 OCA], [http://www.ebi.ac.uk/pdbsum/1vz3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1vz3 RCSB]</span> | ||
}} | }} | ||
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[[Category: Fulop, V.]] | [[Category: Fulop, V.]] | ||
[[Category: Rea, D.]] | [[Category: Rea, D.]] | ||
| - | [[Category: GOL]] | ||
[[Category: alpha/ beta-hydrolase]] | [[Category: alpha/ beta-hydrolase]] | ||
[[Category: amnesia]] | [[Category: amnesia]] | ||
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[[Category: serine protease]] | [[Category: serine protease]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:28:55 2008'' |
Revision as of 21:28, 30 March 2008
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| , resolution 1.6Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | |||||||
| Ligands: | |||||||
| Activity: | Prolyl oligopeptidase, with EC number 3.4.21.26 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
PROLYL OLIGOPEPTIDASE FROM PORCINE BRAIN, T597C MUTANT
Overview
Prolyl oligopeptidase contains a peptidase domain and its catalytic triad is covered by the central tunnel of a seven-bladed beta-propeller. This domain makes the enzyme an oligopeptidase by excluding large structured peptides from the active site. The apparently rigid crystal structure does not explain how the substrate can approach the catalytic groups. Two possibilities of substrate access were investigated: either blades 1 and 7 of the propeller domain move apart, or the peptidase and/or propeller domains move to create an entry site at the domain interface. Engineering disulfide bridges to the expected oscillating structures prevented such movements, which destroyed the catalytic activity and precluded substrate binding. This indicated that concerted movements of the propeller and the peptidase domains are essential for the enzyme action.
About this Structure
1VZ3 is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.
Reference
Concerted structural changes in the peptidase and the propeller domains of prolyl oligopeptidase are required for substrate binding., Szeltner Z, Rea D, Juhasz T, Renner V, Fulop V, Polgar L, J Mol Biol. 2004 Jul 9;340(3):627-37. PMID:15210359
Page seeded by OCA on Mon Mar 31 00:28:55 2008
