1w9d
From Proteopedia
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|PDB= 1w9d |SIZE=350|CAPTION= <scene name='initialview01'>1w9d</scene>, resolution 1.6Å | |PDB= 1w9d |SIZE=350|CAPTION= <scene name='initialview01'>1w9d</scene>, resolution 1.6Å | ||
|SITE= <scene name='pdbsite=AGB:Gol+Binding+Site+For+Chain+M'>AGB</scene> | |SITE= <scene name='pdbsite=AGB:Gol+Binding+Site+For+Chain+M'>AGB</scene> | ||
| - | |LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SEH:S-BENZYL+PHENYLACETOTHIOHYDROXIMATE-O-SULFATE'>SEH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XYP:BETA-D-XYLOPYRANOSE'>XYP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/ | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Thioglucosidase Thioglucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.147 3.2.1.147] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1w9d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w9d OCA], [http://www.ebi.ac.uk/pdbsum/1w9d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1w9d RCSB]</span> | ||
}} | }} | ||
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[[Category: Sinapis alba]] | [[Category: Sinapis alba]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: | + | [[Category: Thioglucosidase]] |
[[Category: Arzt, S.]] | [[Category: Arzt, S.]] | ||
[[Category: Bourderioux, A.]] | [[Category: Bourderioux, A.]] | ||
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[[Category: Rollin, P.]] | [[Category: Rollin, P.]] | ||
[[Category: Tatibouet, A.]] | [[Category: Tatibouet, A.]] | ||
| - | [[Category: GOL]] | ||
| - | [[Category: NAG]] | ||
| - | [[Category: SEH]] | ||
| - | [[Category: SO4]] | ||
| - | [[Category: ZN]] | ||
[[Category: glucotropaeolin]] | [[Category: glucotropaeolin]] | ||
[[Category: glusosinolate]] | [[Category: glusosinolate]] | ||
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[[Category: thiohydroximate]] | [[Category: thiohydroximate]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:33:07 2008'' |
Revision as of 21:33, 30 March 2008
| |||||||
| , resolution 1.6Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | |||||||
| Ligands: | , , , , , , , , | ||||||
| Activity: | Thioglucosidase, with EC number 3.2.1.147 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
S. ALBA MYROSINASE IN COMPLEX WITH S-ETHYL PHENYLACETOTHIOHYDROXIMATE-O-SULFATE
Overview
Myrosinase, a thioglucoside glucohydrolase, is the only enzyme able to hydrolyse glucosinolates, a unique family of molecules bearing an anomeric O-sulfated thiohydroximate function. Non-hydrolysable myrosinase inhibitors have been devised and studied for their biological interaction. Diverse modifications of the O-sulfate moiety did not result in a significant inhibitory effect, whereas replacing the D-glucopyrano residue by its carba-analogue allowed inhibition to take place. X-Ray experiments carried out after soaking allowed for the first time inclusion of a non-hydrolysable inhibitor inside the enzymatic pocket. Structural tuning of the aglycon part in its pocket is being used as a guide for the development of simplified and more potent inhibitors.
About this Structure
1W9D is a Single protein structure of sequence from Sinapis alba. Full crystallographic information is available from OCA.
Reference
The glucosinolate-myrosinase system. New insights into enzyme-substrate interactions by use of simplified inhibitors., Bourderioux A, Lefoix M, Gueyrard D, Tatibouet A, Cottaz S, Arzt S, Burmeister WP, Rollin P, Org Biomol Chem. 2005 May 21;3(10):1872-9. Epub 2005 Apr 14. PMID:15889170
Page seeded by OCA on Mon Mar 31 00:33:07 2008
