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1c8a

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Revision as of 11:13, 24 August 2017

NMR STRUCTURE OF INTRAMOLECULAR DIMER ANTIFREEZE PROTEIN RD3, 40 SA STRUCTURES

Structural highlights

1c8a is a 1 chain structure. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1c89
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ANP3_RHIDE] Contributes to protect fish blood from freezing at subzero sea water temperatures. Lowers the blood freezing point. Binds to nascent ice crystals and prevents further growth (By similarity).

Publication Abstract from PubMed

The structure of a new antifreeze protein (AFP) variant, RD3, from antarctic eel pout (Rhigophila dearborni) with enhanced activity has been determined for the first time by nuclear magnetic resonance spectroscopy. RD3 comprises a unique translational topology of two homologous type III AFP globular domains, each containing one flat, ice binding plane. The ice binding plane of the N domain is located approximately 3.5 A "behind" that of the C domain. The two ice binding planes are located laterally with an angle of 32 +/- 12 degrees between the planes. These results suggest that the C domain plane of RD3 binds first to the ice [1010] prism plane in the <0001> direction, which induces successive ice binding of the N domain in the <0101> direction. This manner of ice binding caused by the unique structural topology of RD3 is thought to be crucial for the significant enhancement of antifreeze activity, especially at low AFP concentrations.

NMR analysis of type III antifreeze protein intramolecular dimer. Structural basis for enhanced activity.,Miura K, Ohgiya S, Hoshino T, Nemoto N, Suetake T, Miura A, Spyracopoulos L, Kondo H, Tsuda S J Biol Chem. 2001 Jan 12;276(2):1304-10. PMID:11010977^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Miura K, Ohgiya S, Hoshino T, Nemoto N, Suetake T, Miura A, Spyracopoulos L, Kondo H, Tsuda S. NMR analysis of type III antifreeze protein intramolecular dimer. Structural basis for enhanced activity. J Biol Chem. 2001 Jan 12;276(2):1304-10. PMID:11010977 doi:10.1074/jbc.M007902200

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1c8a"

@@ Line 1: / Line 1: @@
 ==NMR STRUCTURE OF INTRAMOLECULAR DIMER ANTIFREEZE PROTEIN RD3, 40 SA STRUCTURES==
 <StructureSection load='1c8a' size='340' side='right' caption='[[1c8a]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1c8a]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Antarctic_eel_pout Antarctic eel pout]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C8A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1C8A FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1c8a]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C8A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1C8A FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1c89|1c89]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1c8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c8a OCA], [http://pdbe.org/1c8a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1c8a RCSB], [http://www.ebi.ac.uk/pdbsum/1c8a PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1c8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c8a OCA], [http://pdbe.org/1c8a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1c8a RCSB], [http://www.ebi.ac.uk/pdbsum/1c8a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1c8a ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 24: / Line 25: @@
 __TOC__
 </StructureSection>
-[[Category: Antarctic eel pout]]
 [[Category: Miura, K]]
 [[Category: Tsuda, S]]

1c8a

From Proteopedia

Revision as of 11:13, 24 August 2017

NMR STRUCTURE OF INTRAMOLECULAR DIMER ANTIFREEZE PROTEIN RD3, 40 SA STRUCTURES

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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