1wdc
From Proteopedia
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|PDB= 1wdc |SIZE=350|CAPTION= <scene name='initialview01'>1wdc</scene>, resolution 2.0Å | |PDB= 1wdc |SIZE=350|CAPTION= <scene name='initialview01'>1wdc</scene>, resolution 2.0Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> | + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wdc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wdc OCA], [http://www.ebi.ac.uk/pdbsum/1wdc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1wdc RCSB]</span> | ||
}} | }} | ||
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[[Category: Cohen, C.]] | [[Category: Cohen, C.]] | ||
[[Category: Houdusse, A.]] | [[Category: Houdusse, A.]] | ||
| - | [[Category: CA]] | ||
| - | [[Category: MG]] | ||
[[Category: calcium binding protein]] | [[Category: calcium binding protein]] | ||
[[Category: muscle protein]] | [[Category: muscle protein]] | ||
[[Category: myosin]] | [[Category: myosin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:34:42 2008'' |
Revision as of 21:34, 30 March 2008
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| , resolution 2.0Å | |||||||
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| Ligands: | , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
SCALLOP MYOSIN REGULATORY DOMAIN
Overview
BACKGROUND: In contrast to the myosins of vertebrate skeletal muscle, molluscan myosins are regulated molecules whose enzymatic activity is switched on by the direct binding of Ca2+. The head portion (S1) of the molecule consists of a motor domain and a regulatory domain (RD) containing a 'regulatory' and an 'essential' light chain (RLC and ELC, respectively). The structures of scallop myosin RD with bound Ca2+, as well as the S1 fragment of chicken skeletal muscle myosin, have been determined previously to 2.8 A resolution. RESULTS: We have determined the structure at 2.0 A resolution of scallop myosin RD with bound Ca2+. The unusual coordination at the specific Ca(2+)-binding site in the ELC has now been clarified, as has the structural basis for Mg2+ binding to the RLC. A comparison of the scallop RD structure with that in the chicken S1 structure shows differences in the bending of the two RDs in two different places. CONCLUSIONS: Based on these structural results, a model for regulation is proposed in which the Ca(2+)-bound RD is a rigid structure, and transient flexibility of the Ca(2+)-free RD allows the myosin heads to make stabilizing intramolecular linkage which shut off the motor.
About this Structure
1WDC is a Protein complex structure of sequences from Argopecten irradians. Full crystallographic information is available from OCA.
Reference
Structure of the regulatory domain of scallop myosin at 2 A resolution: implications for regulation., Houdusse A, Cohen C, Structure. 1996 Jan 15;4(1):21-32. PMID:8805510
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