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1es8

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Revision as of 03:57, 6 September 2017

Crystal structure of free BglII

Structural highlights

1es8 is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
NonStd Res:
Related:	1dfm, 1d2i
Activity:	Type II site-specific deoxyribonuclease, with EC number 3.1.21.4
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[T2B2_BACIU] Recognizes the double-stranded sequence AGATCT and cleaves after A-1.

Publication Abstract from PubMed

Restriction endonuclease BglII completely encircles its target DNA, making contacts to both the major and minor grooves. To allow the DNA to enter and leave the binding cleft, the enzyme dimer has to rearrange. To understand how this occurs, we have solved the structure of the free enzyme at 2.3 A resolution, as a complement to our earlier work on the BglII-DNA complex. Unexpectedly, the enzyme opens by a dramatic 'scissor-like' motion, accompanied by a complete rearrangement of the alpha-helices at the dimer interface. Moreover, within each monomer, a set of residues--a 'lever'--lowers or raises to alternately sequester or expose the active site residues. Such an extreme difference in free versus complexed structures has not been reported for other restriction endonucleases. This elegant mechanism for capturing DNA may extend to other enzymes that encircle DNA.

Structure of free BglII reveals an unprecedented scissor-like motion for opening an endonuclease.,Lukacs CM, Kucera R, Schildkraut I, Aggarwal AK Nat Struct Biol. 2001 Feb;8(2):126-30. PMID:11175900^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lukacs CM, Kucera R, Schildkraut I, Aggarwal AK. Structure of free BglII reveals an unprecedented scissor-like motion for opening an endonuclease. Nat Struct Biol. 2001 Feb;8(2):126-30. PMID:11175900 doi:10.1038/84111

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1es8"

@@ Line 1: / Line 1: @@
 ==Crystal structure of free BglII==
 <StructureSection load='1es8' size='340' side='right' caption='[[1es8]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1es8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ES8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ES8 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1es8]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ES8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ES8 FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene></td></tr>
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1dfm|1dfm]], [[1d2i|1d2i]]</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1es8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1es8 OCA], [http://pdbe.org/1es8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1es8 RCSB], [http://www.ebi.ac.uk/pdbsum/1es8 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1es8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1es8 OCA], [http://pdbe.org/1es8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1es8 RCSB], [http://www.ebi.ac.uk/pdbsum/1es8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1es8 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 27: / Line 28: @@
 __TOC__
 </StructureSection>
-[[Category: Bacillus globigii migula 1900]]
 [[Category: Type II site-specific deoxyribonuclease]]
 [[Category: Aggarwal, A K]]

1es8

From Proteopedia

Revision as of 03:57, 6 September 2017

Crystal structure of free BglII

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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