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1gy6

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Revision as of 04:39, 21 September 2017

NTF2 from rat, ammonium sulphate conditions

Structural highlights

1gy6 is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1a2k, 1ar0, 1ask, 1gy5, 1gy7, 1gyb, 1jb2, 1jb4, 1jb5, 1oun, 1qma
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[NTF2_HUMAN] Facilitates protein transport into the nucleus. Interacts with the nucleoporin p62 and with Ran. Acts at a relatively late stage of nuclear protein import, subsequent to the initial docking of nuclear import ligand at the nuclear envelope. Could be part of a multicomponent system of cytosolic factors that assemble at the pore complex during nuclear import.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Interactions with nucleoporins containing FxFG-repeat cores are crucial for the nuclear import of RanGDP mediated by nuclear transport factor 2 (NTF2). We describe here the 1.9 A resolution crystal structure of yeast NTF2-N77Y bound to a FxFG-nucleoporin core, which provides a basis for understanding this interaction and its role in nuclear trafficking. The two identical FxFG binding sites on the dimeric molecule are formed by residues from each chain of NTF2. Engineered mutants at the interaction interface reduce the binding of NTF2 to nuclear pores and cause reduced growth rates and Ran mislocalization when substituted for the wild-type protein in yeast. Comparison with the crystal structure of FG-nucleoporin cores bound to importin-beta and TAP/p15 identified a number of common features of their binding sites. The structure of the binding interfaces on these transport factors provides a rationale for the specificity of their interactions with nucleoporins that, combined with their weak binding constants, facilitates rapid translocation through NPCs during nuclear trafficking.

Structural basis for the interaction between NTF2 and nucleoporin FxFG repeats.,Bayliss R, Leung SW, Baker RP, Quimby BB, Corbett AH, Stewart M EMBO J. 2002 Jun 17;21(12):2843-53. PMID:12065398^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bayliss R, Leung SW, Baker RP, Quimby BB, Corbett AH, Stewart M. Structural basis for the interaction between NTF2 and nucleoporin FxFG repeats. EMBO J. 2002 Jun 17;21(12):2843-53. PMID:12065398 doi:10.1093/emboj/cdf305

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1gy6"

Categories: Bayliss, R | Stewart, M | Nuclear transport

@@ Line 1: / Line 1: @@
-==NTF2 FROM RAT, AMMONIUM SULPHATE CONDITIONS==
+==NTF2 from rat, ammonium sulphate conditions==
 <StructureSection load='1gy6' size='340' side='right' caption='[[1gy6]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1gy6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GY6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GY6 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1gy6]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GY6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GY6 FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1a2k|1a2k]], [[1ar0|1ar0]], [[1ask|1ask]], [[1gy5|1gy5]], [[1gy7|1gy7]], [[1gyb|1gyb]], [[1jb2|1jb2]], [[1jb4|1jb4]], [[1jb5|1jb5]], [[1oun|1oun]], [[1qma|1qma]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gy6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gy6 OCA], [http://pdbe.org/1gy6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gy6 RCSB], [http://www.ebi.ac.uk/pdbsum/1gy6 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gy6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gy6 OCA], [http://pdbe.org/1gy6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gy6 RCSB], [http://www.ebi.ac.uk/pdbsum/1gy6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1gy6 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 16: / Line 17: @@
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
-</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gy6 ConSurf].
 <div style="clear:both"></div>
 <div style="background-color:#fffaf0;">
@@ Line 31: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Buffalo rat]]
 [[Category: Bayliss, R]]
 [[Category: Stewart, M]]
 [[Category: Nuclear transport]]

1gy6

From Proteopedia

Revision as of 04:39, 21 September 2017

NTF2 from rat, ammonium sulphate conditions

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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