1imt

From Proteopedia

(Difference between revisions)

Revision as of 10:39, 27 September 2017

MAMBA INTESTINAL TOXIN 1, NMR, 39 STRUCTURES

Structural highlights

1imt is a 1 chain structure with sequence from Dendroaspis polylepis polylepis. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MIT1_DENPO] Potently contracts gastrointestinal (GI) smooth muscle. The receptor for this toxin is present both in the CNS and in the smooth muscle and may be a potassium channel.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The solution structure of mamba intestinal toxin 1 (MIT1), isolated from Dendroaspis polylepis polylepis venom, has been determined. This molecule is a cysteine-rich polypeptide exhibiting no recognised family membership. Resistance to MIT1 to classical specific endoproteases produced contradictory NMR and biochemical information concerning disulphide-bridge topology. We have used distance restraints allowing ambiguous partners between S atoms in combination with NMR-derived structural information, to correctly determine the disulphide-bridge topology. The resultant solution structure of MIT1, determined to a resolution of 0.5 A, reveals an unexpectedly similar global fold with respect to colipase, a protein involved in fatty acid digestion. Colipase exhibits an analogous resistance to endoprotease activity, indicating for the first time the possible topological origins of this biochemical property. The biochemical and structural homology permitted us to propose a mechanically related digestive function for MIT1 and provides novel information concerning snake venom protein evolution.

A structural homologue of colipase in black mamba venom revealed by NMR floating disulphide bridge analysis.,Boisbouvier J, Albrand JP, Blackledge M, Jaquinod M, Schweitz H, Lazdunski M, Marion D J Mol Biol. 1998;283(1):205-19. PMID:9761684^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Schweitz H, Pacaud P, Diochot S, Moinier D, Lazdunski M. MIT(1), a black mamba toxin with a new and highly potent activity on intestinal contraction. FEBS Lett. 1999 Nov 19;461(3):183-8. PMID:10567694
↑ Boisbouvier J, Albrand JP, Blackledge M, Jaquinod M, Schweitz H, Lazdunski M, Marion D. A structural homologue of colipase in black mamba venom revealed by NMR floating disulphide bridge analysis. J Mol Biol. 1998;283(1):205-19. PMID:9761684 doi:10.1006/jmbi.1998.2057

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1imt"

@@ Line 1: / Line 1: @@
 ==MAMBA INTESTINAL TOXIN 1, NMR, 39 STRUCTURES==
 <StructureSection load='1imt' size='340' side='right' caption='[[1imt]], [[NMR_Ensembles_of_Models | 39 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1imt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Black_mamba Black mamba]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IMT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IMT FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1imt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dendroaspis_polylepis_polylepis Dendroaspis polylepis polylepis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IMT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IMT FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1imt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1imt OCA], [http://pdbe.org/1imt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1imt RCSB], [http://www.ebi.ac.uk/pdbsum/1imt PDBsum]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1imt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1imt OCA], [http://pdbe.org/1imt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1imt RCSB], [http://www.ebi.ac.uk/pdbsum/1imt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1imt ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 15: / Line 16: @@
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
-</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1imt ConSurf].
 <div style="clear:both"></div>
 <div style="background-color:#fffaf0;">
@@ Line 30: / Line 31: @@
 __TOC__
 </StructureSection>
-[[Category: Black mamba]]
+[[Category: Dendroaspis polylepis polylepis]]
 [[Category: Albrand, J P]]
 [[Category: Blackledge, M]]

1imt

From Proteopedia

Revision as of 10:39, 27 September 2017

MAMBA INTESTINAL TOXIN 1, NMR, 39 STRUCTURES

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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