1inh

From Proteopedia

Revision as of 14:34, 5 November 2007

INFLUENZA A SUBTYPE N2 NEURAMINIDASE COMPLEXED WITH AROMATIC BANA111 INHIBITOR

Overview

Influenza virus sialidase is a surface enzyme that is essential for, infection of the virus. The catalytic site is highly conserved among all, known influenza variants, suggesting that this protein is a suitable, target for drug intervention. The most potent known inhibitors are analogs, of 2-deoxy-2,3-didehydro-N-acetylneuraminic acid (Neu5Ac2en), particularly, the 4-guanidino derivative (4-guanidino-Neu5Ac2en). We utilized the, benzene ring of 4-(N-acetylamino)benzoic acids as a cyclic template to, substitute for the dihydropyran ring of Neu5Ac2en. In this study several, 3-(N-acylamino) derivatives were prepared as potential replacements for, the glycerol side chain of Neu5Ac2en, and some were found to interact with, the same binding subsite of sialidase. Of greater significance was the, observation that the 3-guanidinobenzoic acid derivative (equivalent to the, 4-guanidino grouping of 4-guanidino-Neu5Ac2en), the most potent benzoic, acid inhibitor of influenza sialidase thus far identified (IC50 = 10, microM), occupied the glycerol-binding subsite on sialidase as opposed to, the guanidino-binding subsite. This benzoic acid derivative thus provides, a new compound that interacts in a novel manner with the catalytic site of, influenza sialidase.

About this Structure

1INH is a Single protein structure of sequence from Influenza a virus subtype n2 with NAG, CA and ST6 as ligands. Active as Exo-alpha-sialidase, with EC number 3.2.1.18 Structure known Active Sites: CAA and CAB. Full crystallographic information is available from OCA.

Reference

Structure-based inhibitors of influenza virus sialidase. A benzoic acid lead with novel interaction., Singh S, Jedrzejas MJ, Air GM, Luo M, Laver WG, Brouillette WJ, J Med Chem. 1995 Aug 18;38(17):3217-25. PMID:7650674

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