1xd2
From Proteopedia
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|PDB= 1xd2 |SIZE=350|CAPTION= <scene name='initialview01'>1xd2</scene>, resolution 2.70Å | |PDB= 1xd2 |SIZE=350|CAPTION= <scene name='initialview01'>1xd2</scene>, resolution 2.70Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=GDP:GUANOSINE-5'-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= HRAS, HRAS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), SOS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |GENE= HRAS, HRAS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), SOS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1nvv|1NVV]], [[1nvu|1NVU]], [[1nvw|1NVW]], [[1nvx|1NVX]], [[1bkd|1BKD]], [[1xd4|1XD4]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xd2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xd2 OCA], [http://www.ebi.ac.uk/pdbsum/1xd2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1xd2 RCSB]</span> | ||
}} | }} | ||
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==Disease== | ==Disease== | ||
| - | Known | + | Known disease associated with this structure: Fibromatosis, gingival OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=182530 182530]], Noonan syndrome 4 OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=182530 182530]] |
==About this Structure== | ==About this Structure== | ||
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[[Category: Sondermann, H.]] | [[Category: Sondermann, H.]] | ||
[[Category: Yang, S S.]] | [[Category: Yang, S S.]] | ||
| - | [[Category: GDP]] | ||
| - | [[Category: MG]] | ||
| - | [[Category: PO4]] | ||
[[Category: ras-nucleotide exchange factor complex]] | [[Category: ras-nucleotide exchange factor complex]] | ||
[[Category: ras:sos complex]] | [[Category: ras:sos complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:47:55 2008'' |
Revision as of 21:47, 30 March 2008
| |||||||
| , resolution 2.70Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Gene: | HRAS, HRAS1 (Homo sapiens), SOS1 (Homo sapiens) | ||||||
| Related: | 1NVV, 1NVU, 1NVW, 1NVX, 1BKD, 1XD4
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of a ternary Ras:SOS:Ras*GDP complex
Contents |
Overview
The classical model for the activation of the nucleotide exchange factor Son of sevenless (SOS) involves its recruitment to the membrane, where it engages Ras. The recent discovery that Ras*GTP is an allosteric activator of SOS indicated that the regulation of SOS is more complex than originally envisaged. We now present crystallographic and biochemical analyses of a construct of SOS that contains the Dbl homology-pleckstrin homology (DH-PH) and catalytic domains and show that the DH-PH unit blocks the allosteric binding site for Ras and suppresses the activity of SOS. SOS is dependent on Ras binding to the allosteric site for both a lower level of activity, which is a result of Ras*GDP binding, and maximal activity, which requires Ras*GTP. The action of the DH-PH unit gates a reciprocal interaction between Ras and SOS, in which Ras converts SOS from low to high activity forms as Ras*GDP is converted to Ras*GTP by SOS.
Disease
Known disease associated with this structure: Fibromatosis, gingival OMIM:[182530], Noonan syndrome 4 OMIM:[182530]
About this Structure
1XD2 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural analysis of autoinhibition in the Ras activator Son of sevenless., Sondermann H, Soisson SM, Boykevisch S, Yang SS, Bar-Sagi D, Kuriyan J, Cell. 2004 Oct 29;119(3):393-405. PMID:15507210
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