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4xoj

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Revision as of 12:11, 7 October 2017

Structure of bovine trypsin in complex with analogues of sunflower inhibitor 1 (SFTI-1)

Structural highlights

4xoj is a 2 chain structure with sequence from Bos taurus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , , ,
Activity:	Trypsin, with EC number 3.4.21.4
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SFTI1_HELAN] Inhibits trypsin, cathepsin G, elastase, chymotrypsin and thrombin. Does not inhibit factor Xa.^[1]

Publication Abstract from PubMed

Serine proteinase catalyzed peptide splicing was demonstrated in both, one and two-peptide-chain (C- and N-terminal peptide chains linked by a disulfide bridge) trypsin inhibitor SFTI-1 analogues. In the second series, peptide splicing, with catalytic amount of proteinase, was observed only when formation of acyl-enzyme intermediate was preceded by the hydrolysis of the substrate Lys-Ser peptide bond. Here we presented that at an equimolar amount of the proteinase, splicing occurred in all two-peptide-chain analogues. This conclusion was supported by high resolution crystal structures of selected analogues in complex with trypsin. We showed that the process followed a direct transpeptidation mechanism. It means that the acyl-enzyme intermediate was formed and was immediately utilized for a new peptide bond formation, and products associated with the hydrolysis of acyl-enzyme were not observed. The demonstrated peptide splicing was a sequence- not structure-specific.

Investigation of serine proteinase catalyzed peptide splicing in analogues of sunflower trypsin inhibitor 1 (SFTI-1).,Karna N, Malicki S, Debowski D, Golik P, Gitlin A, Grudnik P, Wladyka B, Brzozowski K, Dubin G, Rolka K, Legowska A Chembiochem. 2015 Jul 25. doi: 10.1002/cbic.201500296. PMID:26212347^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Luckett S, Garcia RS, Barker JJ, Konarev AV, Shewry PR, Clarke AR, Brady RL. High-resolution structure of a potent, cyclic proteinase inhibitor from sunflower seeds. J Mol Biol. 1999 Jul 9;290(2):525-33. PMID:10390350 doi:10.1006/jmbi.1999.2891
↑ Karna N, Malicki S, Debowski D, Golik P, Gitlin A, Grudnik P, Wladyka B, Brzozowski K, Dubin G, Rolka K, Legowska A. Investigation of serine proteinase catalyzed peptide splicing in analogues of sunflower trypsin inhibitor 1 (SFTI-1). Chembiochem. 2015 Jul 25. doi: 10.1002/cbic.201500296. PMID:26212347 doi:http://dx.doi.org/10.1002/cbic.201500296

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4xoj"

@@ Line 1: / Line 1: @@
 ==Structure of bovine trypsin in complex with analogues of sunflower inhibitor 1 (SFTI-1)==
 <StructureSection load='4xoj' size='340' side='right' caption='[[4xoj]], [[Resolution|resolution]] 0.91&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xoj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xoj OCA], [http://pdbe.org/4xoj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xoj RCSB], [http://www.ebi.ac.uk/pdbsum/4xoj PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xoj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xoj OCA], [http://pdbe.org/4xoj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xoj RCSB], [http://www.ebi.ac.uk/pdbsum/4xoj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xoj ProSAT]</span></td></tr>
 </table>
 == Function ==

4xoj

From Proteopedia

Revision as of 12:11, 7 October 2017

Structure of bovine trypsin in complex with analogues of sunflower inhibitor 1 (SFTI-1)

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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