1yeh
From Proteopedia
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|PDB= 1yeh |SIZE=350|CAPTION= <scene name='initialview01'>1yeh</scene>, resolution 2.55Å | |PDB= 1yeh |SIZE=350|CAPTION= <scene name='initialview01'>1yeh</scene>, resolution 2.55Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yeh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yeh OCA], [http://www.ebi.ac.uk/pdbsum/1yeh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1yeh RCSB]</span> | ||
}} | }} | ||
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[[Category: Gigant, B.]] | [[Category: Gigant, B.]] | ||
[[Category: Knossow, M.]] | [[Category: Knossow, M.]] | ||
| - | [[Category: ZN]] | ||
[[Category: catalytic antibody]] | [[Category: catalytic antibody]] | ||
[[Category: immunoglobulin c region]] | [[Category: immunoglobulin c region]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:04:48 2008'' |
Revision as of 22:04, 30 March 2008
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| , resolution 2.55Å | |||||||
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| Ligands: | |||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF IGG2A FAB FRAGMENT
Overview
The x-ray structures of the unliganded esterase-like catalytic antibody D2.3 and its complexes with a substrate analogue and with one of the reaction products are analyzed. Together with the structure of the phosphonate transition state analogue hapten complex, these crystal structures provide a complete description of the reaction pathway. At alkaline pH, D2.3 acts by preferential stabilization of the negatively charged oxyanion intermediate of the reaction that results from hydroxide attack on the substrate. A tyrosine residue plays a crucial role in catalysis: it activates the ester substrate and, together with an asparagine, it stabilizes the oxyanion intermediate. A canal allows facile diffusion of water molecules to the reaction center that is deeply buried in the structure. Residues bordering this canal provide targets for mutagenesis to introduce a general base in the vicinity of the reaction center.
About this Structure
1YEH is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
Reference
X-ray structures of a hydrolytic antibody and of complexes elucidate catalytic pathway from substrate binding and transition state stabilization through water attack and product release., Gigant B, Charbonnier JB, Eshhar Z, Green BS, Knossow M, Proc Natl Acad Sci U S A. 1997 Jul 22;94(15):7857-61. PMID:9223277
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