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2ghp

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Revision as of 07:54, 18 October 2017

Crystal structure of the N-terminal 3 RNA binding domains of the yeast splicing factor Prp24

Structural highlights

2ghp is a 8 chain structure with sequence from Atcc 18824. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:
Gene:	PRP24 (ATCC 18824)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT, TOPSAN

Function

[PRP24_YEAST] Binds preferentially to the U4/U6 hybrid snRNAs. Can stimulate the annealing of U4 and U6. Could participate in both the formation and disassembly of the U4/U6 hybrid during splicing.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The essential Saccharomyces cerevisiae pre-messenger RNA splicing protein 24 (Prp24) has four RNA recognition motifs (RRMs) and facilitates U6 RNA base-pairing with U4 RNA during spliceosome assembly. Prp24 is a component of the free U6 small nuclear ribonucleoprotein particle (snRNP) but not the U4/U6 bi-snRNP, and so is thought to be displaced from U6 by U4/U6 base-pairing. The interaction partners of each of the four RRMs of Prp24 and how these interactions direct U4/U6 pairing are not known. Here we report the crystal structure of the first three RRMs and the solution structure of the first two RRMs of Prp24. Strikingly, RRM 2 forms extensive inter-domain contacts with RRMs 1 and 3. These contacts occupy much of the canonical RNA-binding faces (beta-sheets) of RRMs 1 and 2, but leave the beta-sheet of RRM 3 exposed. Previously identified substitutions in Prp24 that suppress mutations in U4 and U6 spliceosomal RNAs cluster primarily in the beta-sheet of RRM 3, but also in a conserved loop of RRM 2. RNA binding assays and chemical shift mapping indicate that a large basic patch evident on the surface of RRMs 1 and 2 is part of a high affinity U6 RNA binding site. Our results suggest that Prp24 binds free U6 RNA primarily with RRMs 1 and 2, which may remodel the U6 secondary structure. The beta-sheet of RRM 3 then influences U4/U6 pairing through interaction with an unidentified ligand.

Structure and interactions of the first three RNA recognition motifs of splicing factor prp24.,Bae E, Reiter NJ, Bingman CA, Kwan SS, Lee D, Phillips GN Jr, Butcher SE, Brow DA J Mol Biol. 2007 Apr 13;367(5):1447-58. Epub 2007 Feb 7. PMID:17320109^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bae E, Reiter NJ, Bingman CA, Kwan SS, Lee D, Phillips GN Jr, Butcher SE, Brow DA. Structure and interactions of the first three RNA recognition motifs of splicing factor prp24. J Mol Biol. 2007 Apr 13;367(5):1447-58. Epub 2007 Feb 7. PMID:17320109 doi:10.1016/j.jmb.2007.01.078

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ghp"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the N-terminal 3 RNA binding domains of the yeast splicing factor Prp24==
 <StructureSection load='2ghp' size='340' side='right' caption='[[2ghp]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PRP24 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ghp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ghp OCA], [http://pdbe.org/2ghp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ghp RCSB], [http://www.ebi.ac.uk/pdbsum/2ghp PDBsum], [http://www.topsan.org/Proteins/CESG/2ghp TOPSAN]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ghp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ghp OCA], [http://pdbe.org/2ghp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ghp RCSB], [http://www.ebi.ac.uk/pdbsum/2ghp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ghp ProSAT], [http://www.topsan.org/Proteins/CESG/2ghp TOPSAN]</span></td></tr>
 </table>
 == Function ==
@@ Line 30: / Line 31: @@
 ==See Also==
-*[[Pre-mRNA-splicing factor|Pre-mRNA-splicing factor]]
 *[[Prp24|Prp24]]
 == References ==

2ghp

From Proteopedia

Revision as of 07:54, 18 October 2017

Crystal structure of the N-terminal 3 RNA binding domains of the yeast splicing factor Prp24

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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